Document Detail


Infection of primary human tonsillar lymphoid cells by KSHV reveals frequent but abortive infection of T cells.
MedLine Citation:
PMID:  21353276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lymphotropic herpesvirus KSHV principally infects B cells in vivo and is linked to several human B cell lymphoproliferative syndromes. Here we examine the susceptibility of primary tonsillar lymphocytes to infection by a recombinant KSHV (rKSHV.219) that constitutively expresses GFP. At an MOI of ~1, ca. 5-10% of CD19+ B cells became GFP-positive. Surprisingly, in the same culture many more T cells became infected. However, in contrast to isolated B cells, isolated infected T cells did not support correct viral transcription and did not produce infectious virus, indicating the presence of one or more post-entry blocks to lytic KSHV replication in T cells. No immortalization or transformation has yet been observed in either B or T cells. These results affirm the feasibility of studying KSHV infection in primary lymphoid cells, and help to rationalize the detection of KSHV DNA in rare human T cell lymphomas in vivo.
Authors:
Jinjong Myoung; Don Ganem
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-02-25
Journal Detail:
Title:  Virology     Volume:  413     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-01     Completed Date:  2011-06-02     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-11     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Cercopithecus aethiops
Herpesviridae Infections / immunology,  virology*
Herpesvirus 8, Human / genetics,  isolation & purification,  physiology*
Humans
Lymphocytes / virology*
Palatine Tonsil / immunology,  virology*
T-Lymphocytes / virology*
Vero Cells
Virus Replication
Grant Support
ID/Acronym/Agency:
R01 CA096491/CA/NCI NIH HHS; R01 CA096491-05/CA/NCI NIH HHS; //Howard Hughes Medical Institute
Comments/Corrections

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