| Infection of primary human tonsillar lymphoid cells by KSHV reveals frequent but abortive infection of T cells. | |
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MedLine Citation:
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PMID: 21353276 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lymphotropic herpesvirus KSHV principally infects B cells in vivo and is linked to several human B cell lymphoproliferative syndromes. Here we examine the susceptibility of primary tonsillar lymphocytes to infection by a recombinant KSHV (rKSHV.219) that constitutively expresses GFP. At an MOI of ~1, ca. 5-10% of CD19+ B cells became GFP-positive. Surprisingly, in the same culture many more T cells became infected. However, in contrast to isolated B cells, isolated infected T cells did not support correct viral transcription and did not produce infectious virus, indicating the presence of one or more post-entry blocks to lytic KSHV replication in T cells. No immortalization or transformation has yet been observed in either B or T cells. These results affirm the feasibility of studying KSHV infection in primary lymphoid cells, and help to rationalize the detection of KSHV DNA in rare human T cell lymphomas in vivo. |
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Authors:
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Jinjong Myoung; Don Ganem |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-02-25 |
Journal Detail:
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Title: Virology Volume: 413 ISSN: 1096-0341 ISO Abbreviation: Virology Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-01 Completed Date: 2011-06-02 Revised Date: 2012-09-24 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 1-11 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Howard Hughes Medical Institute, Departments of Microbiology & Medicine and GW Hooper Foundation, University of California, San Francisco, CA 94143, USA. Jinjong.Myoung@ucsf.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Cercopithecus aethiops Herpesviridae Infections / immunology, virology* Herpesvirus 8, Human / genetics, isolation & purification, physiology* Humans Lymphocytes / virology* Palatine Tonsil / immunology, virology* T-Lymphocytes / virology* Vero Cells Virus Replication |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA096491-05/CA/NCI NIH HHS; //Howard Hughes Medical Institute |
| Comments/Corrections | |
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