Document Detail


Infection of human primary renal epithelial cells with HIV-1 from children with HIV-associated nephropathy.
MedLine Citation:
PMID:  9573536     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Children affected with human immunodefficiency virus (HIV)-associated nephropathy (HIVAN) usually develop significant renal glomerular and tubular epithelial cell injury. The pathogenesis of these changes is not clearly understood. Human renal tubular epithelial cells (RTEc) do not express CD4 surface receptors, and it is not clear whether these cells can be infected by HIV-1. Certain strains of HIV-1, however, have been shown capable of infecting CD4-negative epithelial cell lines. We hypothesized that the inability of laboratory strains of HIV-1 to infect renal epithelial cells may be due to a limited tropism, as opposed to wild-type viruses derived from children with HIVAN, and that viruses derived from these children are capable of infecting RTEc from the same patient. Here, we have demonstrated that HIV-1 isolates from children with HIVAN can productively infect RTEc through a CD4 independent pathway, and that infected mononuclear cells can transfer the virus to human RTEc. Human RTEc sustained low levels of viral replication and HIV-1 inhibited the growth and survival of cultured human RTEc. Thus, HIV-1 may directly induce degenerative changes in RTEc of children with HIVAN. Infected macrophages may play a relevant role in this process by transferring viruses to RTEc.
Authors:
P E Ray; X H Liu; D Henry; L Dye; L Xu; J M Orenstein; T E Schuztbank
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Kidney international     Volume:  53     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-06-30     Completed Date:  1998-06-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1217-29     Citation Subset:  IM; X    
Affiliation:
Children's Research Institute, Children's National Medical Center, Washington, D.C., USA. Pray@cnmc.org
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MeSH Terms
Descriptor/Qualifier:
AIDS-Associated Nephropathy / pathology,  virology*
Cell Division
Cells, Cultured
Child
DNA, Viral / genetics,  isolation & purification
Epithelium / ultrastructure,  virology
HIV-1 / isolation & purification*,  pathogenicity*,  physiology
Humans
In Situ Hybridization
Kidney Tubules / ultrastructure,  virology*
Macrophages / ultrastructure,  virology
Microscopy, Electron
RNA, Viral / genetics,  isolation & purification
Virus Replication
Grant Support
ID/Acronym/Agency:
R0-1 DK49419/DK/NIDDK NIH HHS; R0-1 HL55605/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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