Document Detail


Infection of bovine cells of embryonic origin by amphotropic retroviral vectors.
MedLine Citation:
PMID:  2928839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two amphotropic-based mouse retroviral vectors carrying the neomycin-resistance gene were used to infect four bovine cell lines. Two cell lines, bovine kidney and spleen cells, were refractory to the infection while two independent bovine cells of apparent embryonic origin were infected by the amphotropic retroviral vectors at a measurable titer. Southern blot analysis reveals the presence of neomycin-resistance gene in the G418-resistant bovine cells. The results demonstrate the successful transfer of a gene to bovine cells of embryonic origin using a murine retroviral vector system.
Authors:
L B Zhou; M T Wang; K J Jackson; S M Chang; C B Lawrence
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Somatic cell and molecular genetics     Volume:  15     ISSN:  0740-7750     ISO Abbreviation:  Somat. Cell Mol. Genet.     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-04-25     Completed Date:  1989-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8403568     Medline TA:  Somat Cell Mol Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  137-41     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Southern
Cell Line
Drug Resistance / genetics
Genetic Vectors*
Humans
Neomycin
Plasmids
Retroviridae / genetics*
Transfection / methods
Chemical
Reg. No./Substance:
1404-04-2/Neomycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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