Document Detail

Infection of a Spodoptera frugiperda cell line with Bombyx mori nucleopolyhedrovirus.
MedLine Citation:
PMID:  9085549     Owner:  NLM     Status:  MEDLINE    
The interactions of Bombyx mori nucleopolyhedrovirus (BmNPV) with Spodoptera frugiperda cells (Sf9) were investigated. S. frugiperda cells are usually considered nonpermissive for BmNPV. However, in the present study, BmNPV DNA replication was observed and an increasing infectious titre, reaching 10(4) TCID50/ml on B. mori permissive cells by 6 days post-transfection, developed in the supernatant of infected Sf9 cells. Infection of Sf9 cells by BmNPV did not induce a discernible shutoff of cellular protein synthesis and no overt cytopathic effects were observed. These data indicate that the low permissivity of Sf9 cells for BmNPV replication is associated with an inapparent infection.
O Martin; G Croizier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virus research     Volume:  47     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-07-16     Completed Date:  1997-07-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  179-85     Citation Subset:  IM    
Unité de Génétique des Virus, Station de Recherches de Pathologie Comparée, INRA-URA CNRS 2209, Saint Christol-Lès-Alès, France.
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MeSH Terms
Antigens, Viral / analysis
Bombyx / virology*
Cell Line
DNA Replication
DNA, Viral
Nucleopolyhedrovirus / genetics,  growth & development,  physiology*
Spodoptera / cytology
Virus Replication
Reg. No./Substance:
0/Antigens, Viral; 0/DNA, Viral

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