Document Detail


Infection of a Spodoptera frugiperda cell line with Bombyx mori nucleopolyhedrovirus.
MedLine Citation:
PMID:  9085549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The interactions of Bombyx mori nucleopolyhedrovirus (BmNPV) with Spodoptera frugiperda cells (Sf9) were investigated. S. frugiperda cells are usually considered nonpermissive for BmNPV. However, in the present study, BmNPV DNA replication was observed and an increasing infectious titre, reaching 10(4) TCID50/ml on B. mori permissive cells by 6 days post-transfection, developed in the supernatant of infected Sf9 cells. Infection of Sf9 cells by BmNPV did not induce a discernible shutoff of cellular protein synthesis and no overt cytopathic effects were observed. These data indicate that the low permissivity of Sf9 cells for BmNPV replication is associated with an inapparent infection.
Authors:
O Martin; G Croizier
Related Documents :
14738979 - Development of an in situ hybridisation procedure for the detection of sole aquabirnavi...
6195529 - Association of human t-cell leukaemia/lymphoma virus with the tac antigen marker for th...
7903669 - Organization of the double-stranded rna-activated protein kinase dai and virus-associat...
17336569 - Aberrant life cycle of human immunodeficiency virus type 1 crf15_01b-like clinical isol...
18923829 - Baculovirus production for gene therapy: the role of cell density, multiplicity of infe...
403139 - In vitro interaction of neisseria gonorrhoeae type 1 and type 4 with tissue culture cells.
2843519 - Identification and characterization of three distinct atrial natriuretic factor recepto...
6299929 - Ovarian substance induces steroid production in cultured granulosa cells.
9685659 - Dielectric behavior of budding yeast in cell separation.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virus research     Volume:  47     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-07-16     Completed Date:  1997-07-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  179-85     Citation Subset:  IM    
Affiliation:
Unité de Génétique des Virus, Station de Recherches de Pathologie Comparée, INRA-URA CNRS 2209, Saint Christol-Lès-Alès, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Viral / analysis
Bombyx / virology*
Cell Line
DNA Replication
DNA, Viral
Nucleopolyhedrovirus / genetics,  growth & development,  physiology*
Rabbits
Spodoptera / cytology
Transfection
Virus Replication
Chemical
Reg. No./Substance:
0/Antigens, Viral; 0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comparison of the complete nucleotide sequences of two isolates of lettuce mosaic virus differing in...
Next Document:  Antigenic diversity of Akabane virus detected by monoclonal antibodies.