Document Detail


Infection and propagation of human rhinovirus C in human airway epithelial cells.
MedLine Citation:
PMID:  23035218     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human rhinovirus species C (HRV-C) was recently discovered using molecular diagnostic techniques and is associated with lower respiratory tract disease, particularly in children. HRV-C cannot be propagated in immortalized cell lines, and currently sinus organ culture is the only system described that is permissive to HRV-C infection ex vivo. However, the utility of organ culture for studying HRV-C biology is limited. Here, we report that a previously described HRV-C derived from an infectious cDNA, HRV-C15, infects and propagates in fully differentiated human airway epithelial cells but not in undifferentiated cells. We demonstrate that this differentiated epithelial cell culture system supports infection and replication of a second virus generated from a cDNA clone, HRV-C11. We show that HRV-C15 virions preferentially bind fully differentiated airway epithelial cells, suggesting that the block to replication in undifferentiated cells is at the step of viral entry. Consistent with previous reports, HRV-C15 utilizes a cellular receptor other than ICAM-1 or LDLR for infection of differentiated epithelial cells. Furthermore, we demonstrate that HRV-C15 replication can be inhibited by an HRV 3C protease inhibitor (rupintrivir) but not an HRV capsid inhibitor previously under clinical development (pleconaril). The HRV-C cell culture system described here provides a powerful tool for studying the biology of HRV-C and the discovery and development of HRV-C inhibitors.
Authors:
Weidong Hao; Katie Bernard; Nita Patel; Nancy Ulbrandt; Hui Feng; Catherine Svabek; Susan Wilson; Christina Stracener; Kathy Wang; Joann Suzich; Wade Blair; Qing Zhu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-03
Journal Detail:
Title:  Journal of virology     Volume:  86     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-20     Completed Date:  2013-01-28     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13524-32     Citation Subset:  IM    
Affiliation:
Department of Infectious Diseases and Vaccines, MedImmune LLC, Gaithersburg, Maryland, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Bronchi / cytology,  virology*
Cell Differentiation
DNA Primers
Epithelial Cells / virology
HeLa Cells
Humans
Male
Middle Aged
Picornaviridae Infections / pathology,  virology*
Polymerase Chain Reaction
Rhinovirus / physiology*
Virus Replication*
Chemical
Reg. No./Substance:
0/DNA Primers
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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