Document Detail

Infection of Nonphagocytic Host Cells by Legionella.
MedLine Citation:
PMID:  23150414     Owner:  NLM     Status:  Publisher    
Legionella pneumophila is an intracellular pathogen of free-living protozoa that can also infect alveolar macrophages, L929 fibroblast cells, and HeLa cells. Infection of nonphagocytic cells by L. pneumophila can be used to study invasion mechanisms, compare infectivity of different strains and identify factors important for virulence. Virulent strains of L. pneumophila exposed to monolayers of L929 cells are able to invade and form virus-like plaques, which can be enumerated as a measure of infectivity. Invasiveness of HeLa cells can also be used to evaluate relative infectivity and to study mechanisms of invasion and to track the development of cyst-like forms. The detailed methods of both the L929 plaque assay and HeLa cell invasion assay are described.
Shandra R Day; Costi D Sifri; Paul S Hoffman
Related Documents :
23876874 - Next-generation molecular-diagnostic tools for gastrointestinal nematodes of livestock,...
22633394 - Tissue expander with acellular dermal matrix for breast reconstruction infected by an u...
24336244 - The effect of hormones on the lower urinary tract.
22337824 - The dilemma of assessment bias in infection control research.
21979784 - Gastrointestinal nematode infections in german sheep.
20568674 - Polyomavirus bk neutralizing activity in human immunoglobulin preparations.
Publication Detail:
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  954     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  463-478     Citation Subset:  -    
Division of Infectious Diseases and International Health, University of Virginia Health System, Charlottesville, VA, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The Caenorhabditis elegans Model of Legionella Infection.
Next Document:  Analyzing Caspase-1 Activation During Legionella pneumophila Infection in Macrophages.