| Infection of Nonphagocytic Host Cells by Legionella. | |
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MedLine Citation:
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PMID: 23150414 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Legionella pneumophila is an intracellular pathogen of free-living protozoa that can also infect alveolar macrophages, L929 fibroblast cells, and HeLa cells. Infection of nonphagocytic cells by L. pneumophila can be used to study invasion mechanisms, compare infectivity of different strains and identify factors important for virulence. Virulent strains of L. pneumophila exposed to monolayers of L929 cells are able to invade and form virus-like plaques, which can be enumerated as a measure of infectivity. Invasiveness of HeLa cells can also be used to evaluate relative infectivity and to study mechanisms of invasion and to track the development of cyst-like forms. The detailed methods of both the L929 plaque assay and HeLa cell invasion assay are described. |
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Authors:
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Shandra R Day; Costi D Sifri; Paul S Hoffman |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 954 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: - |
Other Details:
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Languages: ENG Pagination: 463-478 Citation Subset: - |
Affiliation:
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Division of Infectious Diseases and International Health, University of Virginia Health System, Charlottesville, VA, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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