Document Detail

Infection, Multiplication and Evaluation of the Nitrogen-Fixing Ability of Herbaspirillum sp. Strain B501gfp1 in Sugarcane Stems Inoculated by the Vacuum Infiltration Method.
MedLine Citation:
PMID:  21558698     Owner:  NLM     Status:  In-Data-Review    
The present study was conducted with the aim of finding a suitable delivery method for introducing endophytic bacteria into new sugarcane stalks. Stem pieces of cultivars NiF8 and Ni15 were inoculated with Herbaspirillum sp. strain B501gfp1 by the vacuum infiltration method. The inoculated bacteria showed higher levels of nitrogenase activity in cultivar Ni15 than NiF8. The population density immediately after inoculation (10(7) CFU g [fresh weight](-1)) showed that strain B501gfp1 successfully entered the stem tissues of Ni15, where its presence was confirmed by fluorescence microscopic observations. Colonization was observed in the intercellular spaces and between the cell layers. The population of inoculated bacteria remained stable at 5 days after inoculation. The inoculated stems also showed adequately strong acetylene reduction activity compared to uninoculated stems in the absence of indigenous nitrogen-fixing bacteria.
Muhammad Zakria; Kana Udonishi; Yuichi Saeki; Akihiro Yamamoto; Shoichiro Akao
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbes and environments / JSME     Volume:  23     ISSN:  1342-6311     ISO Abbreviation:  Microbes Environ.     Publication Date:  2008  
Date Detail:
Created Date:  2011-05-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9710937     Medline TA:  Microbes Environ     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  128-33     Citation Subset:  -    
Faculty of Agriculture, University of Miyazaki.
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