Document Detail

Induction of vascular adhesion protein-1 during liver allograft rejection and concomitant cytomegalovirus infection in rats.
MedLine Citation:
PMID:  11021827     Owner:  NLM     Status:  MEDLINE    
Vascular adhesion protein-1 (VAP-1) is an adhesion molecule controlling lymphocyte recirculation through high endothelial venules of the lymph nodes. It has also been shown to be induced and to mediate lymphocyte adhesion at sites of inflammation. We studied the expression of VAP-1 and two other inducible adhesion molecules ICAM-1 and VCAM-1 in our experimental model of rat liver allograft rejection and, in addition, the effect of concomitant rat cytomegalovirus (RCMV) infection on this expression. Expression of VAP-1, ICAM-1, and VCAM-1 was studied in rat liver allografts with or without RCMV infection, isografts, and normal rat liver. Immunoperoxidase technique and monoclonal antibodies including a novel anti-VAP-1 reagent were used. VAP-1 expression was induced by acute rejection in sinusoids, hepatocytes, and also in bile ducts, when compared to the isografts or normal liver, where only blood vessels were consistently positive. Sinusoidal and hepatocyte expression of VAP-1 was prolonged by the presence of RCMV. ICAM-1 and VCAM-1 expression was also induced by acute rejection. However, RCMV increased sinusoidal VCAM-1 expression compared to uninfected grafts. The present experimental study shows that VAP-1 is up-regulated in acute rejection of liver allografts, and that this up-regulation is prolonged by RCMV infection.
T Martelius; M Salmi; H Wu; C Bruggeman; K Höckerstedt; S Jalkanen; I Lautenschlager
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of pathology     Volume:  157     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-10-16     Completed Date:  2000-10-25     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1229-37     Citation Subset:  AIM; IM    
Departments of Surgery and Virology, Helsinki University Hospital, and University of Helsinki, Helsinki, Finland.
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MeSH Terms
Acute Disease
Amine Oxidase (Copper-Containing) / genetics,  metabolism*
CHO Cells
Cell Adhesion Molecules / genetics,  metabolism*
Cytomegalovirus Infections / complications*,  metabolism*
Graft Rejection / complications*,  metabolism*
Intercellular Adhesion Molecule-1 / metabolism
Liver / metabolism
Liver Transplantation*
RNA, Messenger / metabolism
Rats, Inbred BN
Rats, Inbred Strains
Reference Values
Time Factors
Transplantation, Homologous
Transplantation, Isogeneic
Vascular Cell Adhesion Molecule-1 / metabolism
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/RNA, Messenger; 0/Vascular Cell Adhesion Molecule-1; 126547-89-5/Intercellular Adhesion Molecule-1; EC protein, human; EC Oxidase (Copper-Containing)

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