Document Detail


Induction of trophoblast cell fusion by a protein tyrosine phosphatase inhibitor.
MedLine Citation:
PMID:  18076988     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the placenta, trophoblast cell fusion leads to the formation of the syncytiotrophoblast, which plays an essential role in the diffusion of nutrients and hormones from the mother to the fetus. Different protein tyrosine kinases are involved in the modulation of this biological process. Herein, we investigated the impact of a protein tyrosine phosphatase (PTP) inhibitor (bpV[pic]) on trophoblast fusion. Upon bpV[pic] or forskolin treatment, primary and BeWo trophoblastic cells demonstrated higher cAMP levels and a more potent induction of cell fusion, while non-fusogenic JEG-3 cells were non-responsive to these treatments. RT-PCR analyses on stimulated BeWo cells or primary cytotrophoblast cells demonstrated an increase in syncytin-1 mRNA levels, which was more pronounced upon forskolin/bpV[pic] treatment. Using the luciferase gene upstream of the syncytin-1 promoter, similar results were obtained in BeWo cells after stimulation. These results demonstrate that PTPs act negatively on cell fusion in human trophoblasts and could control the timing of syncytiotrophoblast formation during placenta development.
Authors:
A Vargas; J Moreau; F Le Bellego; J Lafond; B Barbeau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Placenta     Volume:  29     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-05     Completed Date:  2008-05-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  170-4     Citation Subset:  IM    
Affiliation:
Département des Sciences Biologiques and BioMed Research Center, Université du Québec à Montréal, 2080 St-Urbain, Montréal, QC, H2X 3X8, Canada.
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MeSH Terms
Descriptor/Qualifier:
Cell Fusion
Cells, Cultured
Enzyme Inhibitors / pharmacology
Female
Gene Products, env / genetics
Humans
Luciferases / genetics
Organometallic Compounds / pharmacology*
Pregnancy
Pregnancy Proteins / genetics
Promoter Regions, Genetic / physiology
Protein Tyrosine Phosphatases / antagonists & inhibitors*,  physiology
Recombinant Proteins / genetics
Transfection
Trophoblasts / drug effects*,  physiology*
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Gene Products, env; 0/Organometallic Compounds; 0/Pregnancy Proteins; 0/Recombinant Proteins; 0/oxodiperoxo(pyridine-2-carboxylate)vanadate(V); 0/syncytin; EC 1.13.12.-/Luciferases; EC 3.1.3.48/Protein Tyrosine Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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