Document Detail


Induction to maturation of the sea urchin Paracentrotus lividus (Lamarck, 1816) under laboratory conditions.
MedLine Citation:
PMID:  20088209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Laboratory experiments were carried out over two different periods, with the aim of investigating and utilizing the induction to gonadal maturation of the sea urchin Paracentrotus lividus. The final objective was to obtain viable gametes outside the period of natural spawning in the environment; which would allow the utilization of ecotoxicological bioassays with sea urchin larvae at any time of the year. The experiment consisted of maintaining some sea urchins in tanks and providing them with a natural photoperiod, unlimited food and a constant temperature of 20 degrees C. During days 0, 30, 60 and 90, gonads from 15 of these sea urchins were compared with those collected simultaneously in the natural environment. The gametes obtained were used to carry out fecundations, in order to check their viability. The final results obtained were clearly influenced by the gonadal state of the sea urchins at the initial stage of the experiment. The best results were obtained within a time period of 60 days and when the initial gonad index was low.
Authors:
Joxe Mikel Garmendia; Iratxe Menchaca; Mar?a Jes?s Belzunce; Javier Franco; Marta Revilla
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental technology     Volume:  30     ISSN:  0959-3330     ISO Abbreviation:  Environ Technol     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-02-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9884939     Medline TA:  Environ Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1441-6     Citation Subset:  IM    
Affiliation:
AZTI - Tecnalia, Herrera kaia portualdea z/g, 20110 Pasaia, Spain. jgarmendia@azti.es
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / methods*
Cell Survival
Gonads / anatomy & histology,  growth & development
Organ Size
Paracentrotus / anatomy & histology,  growth & development*
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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