| Induction of system A amino acid transport through long-term treatment with ouabain: correlation with increased (Na+/K+)-ATPase activity. | |
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MedLine Citation:
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PMID: 2836438 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mouse embryo fibroblast cells (C3H-10T1/2) and the methylcholanthrene-transformed derivative (MCA-10T1/2) were treated with basal modified Eagle's medium (BME) containing 10% fetal bovine serum and varying concentrations of ouabain ranging from 0.05 mM to 0.7 mM for 16 h in culture. After replacing the ouabain-containing medium with Earl's balanced salts solution, System A amino acid transport activity increased from approximately 40 to 500 pmol AIB accumulated.mg protein-1.min-1 in the C3H-10T1/2 cells and from approximately 300 to 700 pmol AIB accumulated.mg protein-1.min-1 in the MCA-10T1/2 cells. The (Na+/K+)-ATPase pump activity also increased from approximately 12 to 46 nmol Rb+ accumulated.mg protein-1.min-1 in the normal cells and from approximately 20 to 42 nmol Rb+ accumulated.mg protein-1.min-1 in the transformed cells. System A and the (Na+/K+)ATPase activity were maximally increased at approximately 0.4-0.6 mM ouabain in the normal cells in contrast to the transformed cells which were maximally stimulated at a concentration of approximately 0.2 mM ouabain. This treatment with ouabain increased the [Na+]i/[K+]i as measured by atomic absorption spectroscopy, and thereby decreased the Na+ and K+ electrochemical gradients. Our data show that the internal ion gradients inverted at a lower concentration of ouabain in the transformed cells compared to the normal cells. The ouabain-induced increase in pump and System A activity shown here was used as a tool to further investigate the coordinated ion transport regulation in the control of cell growth. |
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Authors:
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M A Schenerman; K J Leister; D K Trachtenberg; E Racker |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 135 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1988 May |
Date Detail:
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Created Date: 1988-07-05 Completed Date: 1988-07-05 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 157-62 Citation Subset: IM |
Affiliation:
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Section of Biochemistry, Molecular, and Cell Biology, Cornell University, Ithaca, New York 14853. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism* Aminoisobutyric Acids / metabolism Animals Biological Transport / drug effects Cells, Cultured Kinetics Mice Ouabain / pharmacology* Potassium / metabolism Rubidium / metabolism Sodium / metabolism Sodium-Potassium-Exchanging ATPase / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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CA-08277/CA/NCI NIH HHS; CA-08964/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Aminoisobutyric Acids; 62-57-7/2-aminoisobutyric acid; 630-60-4/Ouabain; 7440-09-7/Potassium; 7440-17-7/Rubidium; 7440-23-5/Sodium; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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