Document Detail

Induction of system A amino acid transport through long-term treatment with ouabain: correlation with increased (Na+/K+)-ATPase activity.
MedLine Citation:
PMID:  2836438     Owner:  NLM     Status:  MEDLINE    
Mouse embryo fibroblast cells (C3H-10T1/2) and the methylcholanthrene-transformed derivative (MCA-10T1/2) were treated with basal modified Eagle's medium (BME) containing 10% fetal bovine serum and varying concentrations of ouabain ranging from 0.05 mM to 0.7 mM for 16 h in culture. After replacing the ouabain-containing medium with Earl's balanced salts solution, System A amino acid transport activity increased from approximately 40 to 500 pmol AIB protein-1.min-1 in the C3H-10T1/2 cells and from approximately 300 to 700 pmol AIB protein-1.min-1 in the MCA-10T1/2 cells. The (Na+/K+)-ATPase pump activity also increased from approximately 12 to 46 nmol Rb+ protein-1.min-1 in the normal cells and from approximately 20 to 42 nmol Rb+ protein-1.min-1 in the transformed cells. System A and the (Na+/K+)ATPase activity were maximally increased at approximately 0.4-0.6 mM ouabain in the normal cells in contrast to the transformed cells which were maximally stimulated at a concentration of approximately 0.2 mM ouabain. This treatment with ouabain increased the [Na+]i/[K+]i as measured by atomic absorption spectroscopy, and thereby decreased the Na+ and K+ electrochemical gradients. Our data show that the internal ion gradients inverted at a lower concentration of ouabain in the transformed cells compared to the normal cells. The ouabain-induced increase in pump and System A activity shown here was used as a tool to further investigate the coordinated ion transport regulation in the control of cell growth.
M A Schenerman; K J Leister; D K Trachtenberg; E Racker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  135     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-07-05     Completed Date:  1988-07-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  157-62     Citation Subset:  IM    
Section of Biochemistry, Molecular, and Cell Biology, Cornell University, Ithaca, New York 14853.
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MeSH Terms
Amino Acids / metabolism*
Aminoisobutyric Acids / metabolism
Biological Transport / drug effects
Cells, Cultured
Ouabain / pharmacology*
Potassium / metabolism
Rubidium / metabolism
Sodium / metabolism
Sodium-Potassium-Exchanging ATPase / metabolism*
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/Aminoisobutyric Acids; 62-57-7/2-aminoisobutyric acid; 630-60-4/Ouabain; 7440-09-7/Potassium; 7440-17-7/Rubidium; 7440-23-5/Sodium; EC ATPase

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