Document Detail


Induction of syncytia by neuropathogenic murine leukemia viruses depends on receptor density, host cell determinants, and the intrinsic fusion potential of envelope protein.
MedLine Citation:
PMID:  10516046     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infection by the neuropathogenic murine leukemia virus (MLV) TR1.3 results in hemorrhagic disease that correlates directly to in vivo syncytium formation of brain capillary endothelial cells (BCEC). This phenotype maps to amino acid 102 in the envelope (Env) protein of TR1.3. Substitution of glycine (G) for tryptophan (W) at this position (W102G Env) in the nonpathogenic MLV FB29 induces both syncytium formation and neurologic disease in vivo. Using an in vitro gene reporter cell fusion assay, we showed that fusion either with murine NIH 3T3 cells or with nonmurine target cells that expressed receptors at or below endogenous murine levels mirrored that seen in BCEC in vivo. In these instances only TR1.3 and W102G Env induced cell fusion. In contrast, when receptor levels on nonmurine cells were raised above endogenous murine levels, FB29 Env was as fusogenic as the neuropathogenic TR1.3 and W102G Env. These results indicate that TR1.3 Env and W102G Env are intrinsically more fusogenic than FB29 Env, that the induction of fusion requires a threshold number of receptors that is greater for FB29 Env than for TR1.3 or W102G Env, and that receptor density on murine NIH 3T3 cells and BCEC is below the threshold for FB29-dependent fusion. Surprisingly, receptor density on NIH 3T3 cells could not be increased by stable expression of exogenous receptors, and FB29-dependent fusion was not observed in NIH 3T3 cells that transiently expressed elevated receptor numbers. These results suggest that an additional undefined host cell factor(s) may limit both receptor expression and fusion potential in murine cells.
Authors:
M Chung; K Kizhatil; L M Albritton; G N Gaulton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  73     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-11-04     Completed Date:  1999-11-04     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9377-85     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Cell Fusion
Cell Line
Coturnix
Genes, Reporter
Giant Cells / physiology*
Humans
Leukemia Virus, Murine / pathogenicity*,  physiology*
Membrane Fusion
Mice
Receptors, Virus / metabolism
Viral Envelope Proteins / metabolism*
Virulence
Grant Support
ID/Acronym/Agency:
AI33410/AI/NIAID NIH HHS; NS30606/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Virus; 0/Viral Envelope Proteins
Comments/Corrections

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