| Induction of spermatogenic cell apoptosis in prepubertal rat testes irrespective of testicular steroidogenesis: a possible estrogenic effect of di(n-butyl) phthalate. | |
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MedLine Citation:
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PMID: 19903717 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although di(n-butyl) phthalate (DBP), a suspected endocrine disruptor, induces testicular atrophy in prepubertal male rats, whether it exerts estrogenic activity in vivo remains a matter of debate. In the present study, we explored the estrogenic potency of DBP using 3-week-old male rats, and then examined the relationship between estrogen-induced spermatogenic cell apoptosis and testicular steroidogenesis. Daily exposure to DBP for 7 days caused testicular atrophy due to loss of spermatogenic cells, whereas testicular steroidogenesis was almost the same with the control values. A single exposure of DBP decreased testicular steroidogenesis in addition to decreasing the level of serum LH at 3 h after DBP treatment, with an extremely high incidence of apoptotic spermatogenic cells at 6 h after administration. To elucidate the estrogenic activity of DBP, we carried out an inhibition study using pure antiestrogen ICI 182,780 (ICI) in a model of spermatogenic cell apoptosis induced by DBP or estradial-3-benzoate (EB). Although both the DBP- and EB-treated groups showed a significant increase in spermatogenic cell apoptosis, ICI pretreatment significantly decreased the number of apoptotic spermatogenic cells in these two groups. In contrast, testicular steroidogenesis and serum FSH were significantly reduced in all the treated groups, even in the DBP+ICI and EB+ICI groups. Taken together, these findings led us to conclude that estrogenic compounds such as DBP and EB induce spermatogenic cell apoptosis in prepubertal rats, probably by activating estrogen receptors in testis, and that reduction in testicular steroidogenic function induced by estrogenic compounds is not associated with spermatogenic cell apoptosis. |
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Authors:
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Mohammad Shah Alam; Seiichiroh Ohsako; Takashi Matsuwaki; Xiao Bo Zhu; Naoki Tsunekawa; Yoshiakira Kanai; Hideko Sone; Chiharu Tohyama; Masamichi Kurohmaru |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-10 |
Journal Detail:
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Title: Reproduction (Cambridge, England) Volume: 139 ISSN: 1741-7899 ISO Abbreviation: Reproduction Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-26 Completed Date: 2010-02-22 Revised Date: 2013-06-11 |
Medline Journal Info:
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Nlm Unique ID: 100966036 Medline TA: Reproduction Country: England |
Other Details:
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Languages: eng Pagination: 427-37 Citation Subset: IM |
Affiliation:
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Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Atrophy Dibutyl Phthalate / toxicity* Dose-Response Relationship, Drug Endocrine Disruptors / toxicity* Estradiol / analogs & derivatives, pharmacology, toxicity Estrogen Antagonists / pharmacology Estrogens / toxicity* Follicle Stimulating Hormone / blood Leydig Cells / drug effects*, pathology Luteinizing Hormone / blood Male Rats Sexual Maturation Spermatogenesis / drug effects* Testis / drug effects*, growth & development, metabolism, pathology Testosterone / biosynthesis* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Endocrine Disruptors; 0/Estrogen Antagonists; 0/Estrogens; 1S4CJB5ZGN/estradiol 3-benzoate; 22X328QOC4/fulvestrant; 50-28-2/Estradiol; 58-22-0/Testosterone; 84-74-2/Dibutyl Phthalate; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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