| Induction of specific gelatinolytic proteinases in the lateral prostate of rats by ectopic pituitary grafts. | |
| | |
MedLine Citation:
|
PMID: 1576265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Activities of plasminogen activator (PA) and gelatinolytic proteinase in the ventral, lateral, and dorsal lobes of the prostate were examined in rats with hyperprolactinemia, which was induced by implantation of ectopic pituitary grafts under the renal capsule. Weight and total DNA content of the lateral lobe increased significantly in rats receiving two pituitary glands as compared with values for the initial untreated group and the muscle-implanted control animals. No change in specific activity of PA was observed in any prostatic lobe for animals of different treatment groups. However, there was a lateral lobe-specific induction of gelatinolytic proteinase activity in pituitary-grafted rats. Two Ca(2+)-independent proteinase activities of approximately 26-28 and 88 kDa, and three calcium-sensitive proteinase activities of 59, 64, and 76 kDa were found in zymograms of lateral prostate extracts of the untreated initial group and the muscle-grafted control. The lateral prostate proteinase pattern of pituitary-grafted animals demonstrated intense activities of approximately 51, 53, 64, 76, and 135 kDa as well as weaker activities of 40, 43, 59, 85, 93, 105, and 125 kDa. Many of these proteinase activities were present in the secretion of the lateral prostate of the untreated initial controls, suggesting that hyperprolactinemia promotes secretion in the lateral prostate. Those proteinase activities that were not in the secretion were probably of intracellular origin and may play a role in cellular remodeling. Castration resulted in a decrease in activities of these proteinases, and this decrease was observed whether the pituitary grafts were retained or removed. |
| | |
Authors:
|
C Lee; J A Sensibar; A A Sinha; M J Wilson |
Related Documents
:
|
11383885 - Differential localization of prohormone convertases pc1 and pc2 in two distinct types o... 1855455 - Differentiated metabolic stimulation of rat pituitary lobes by peripheral and central e... 3736785 - Enkephalin biosynthesis and processing during lactation. 16543265 - Novel role of 26rfa, a hypothalamic rfamide orexigenic peptide, as putative regulator o... 6299515 - Effects of chrysotile asbestos on lung and pleural carcinogenesis of n-bis(2-hydroxypro... 6930475 - Experimental gingivitis in odu plaque-susceptible rats. v. the presence of bradykinin i... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biology of reproduction Volume: 46 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1992 Apr |
Date Detail:
|
Created Date: 1992-06-11 Completed Date: 1992-06-11 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 671-9 Citation Subset: IM |
Affiliation:
|
Department of Urology, Northwestern University Medical School, Chicago, Illinois 60611. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Choristoma* DNA / analysis Endopeptidases / biosynthesis* Enzyme Induction / physiology Hyperprolactinemia / physiopathology Male Metalloendopeptidases / biosynthesis Organ Size / physiology Pituitary Gland* Plasminogen Activators / biosynthesis Prolactin / pharmacology Prostate / anatomy & histology, chemistry, enzymology* Prostatic Neoplasms / enzymology* Rats Rats, Inbred Strains Testosterone / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
DK39250/DK/NIDDK NIH HHS; HD11611/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
58-22-0/Testosterone; 9002-62-4/Prolactin; 9007-49-2/DNA; EC 3.4.-/Endopeptidases; EC 3.4.21.-/Plasminogen Activators; EC 3.4.24.-/Metalloendopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Epostane and indomethacin actions on ovarian kallikrein and plasminogen activator activities during ...
Next Document: Effects of endothelin and relaxin on rat uterine segment contractility.