Document Detail

Induction of salt tolerance in Bacillus subtilis IFO 3025.
MedLine Citation:
PMID:  16233506     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Salt tolerance was induced in Bacillus subtilis IFO 3025 cells when a moderate osmotic stress was imposed by incubation in the presence of compatible solutes and 0.5 M NaCl or 0.8 M sorbitol. The optimum condition for inducing salt tolerance was observed when the cells were incubated in a solution containing 5 mM glutamate, 2.5 mM KCl, and 0.5 M NaCl or 0.8 M sorbitol. After 60 min incubation, the cells were able to form colonies on an agar plate with high salinity. It was confirmed that the cells acquired salt tolerance by accumulating glutamate and potassium ions in the cytoplasm as the main solute and ion, respectively.
Tomohiko Ikeuchi; Akio Ishida; Masayuki Tajifi; Shinichi Nagata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  96     ISSN:  1389-1723     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2003  
Date Detail:
Created Date:  2005-10-19     Completed Date:  2005-11-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  184-6     Citation Subset:  -    
Research Institute for Marine Cargo Transportation, Kobe University of Mercantile Marine, 5-1-1 Fukae, Higashinada-ku, Kobe 658-0022, Japan.
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