Document Detail

Induction and repair of (6-4) photoproducts in normal human and xeroderma pigmentosum variant cells during the cell cycle.
MedLine Citation:
PMID:  7491119     Owner:  NLM     Status:  MEDLINE    
The reduced rate of (6-4) photoproduct repair observed in some cell lines may represent a more severe repair deficiency in some cohort of the cell cycle, such as S-phase. Radioimmunoassay was used to determine the kinetics of (6-4) photoproduct repair in normal human fibroblasts and xeroderma pigmentosum variant cells fractionated into different phases of the cell cycle by counterflow centrifugal elutriation. Ultraviolet fluence response curves indicated that the same amount of (6-4) photoproduct damage was induced at all phases of the cell cycle. The extent of (6-4) photoproduct repair in asynchronous XP variant cells was significantly reduced compared to normal human cells. However, the rate and extent of (6-4) photoproduct repair was constant throughout the cell cycle in both normal and XP variant cells. Hence, the UV hypersensitive and hypermutable phenotypes observed in XP variant cells are not attributable to cell cycle-dependent deficiencies in excision repair nor the yield of photodamage through the cell cycle.
D L Mitchell; J E Cleaver; M P Lowery; R R Hewitt
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Mutation research     Volume:  337     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-01-02     Completed Date:  1996-01-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  161-7     Citation Subset:  IM    
University of Texas M.D. Anderson Cancer Center, Smithville 78957, USA.
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MeSH Terms
Cell Cycle / genetics*
Cell Separation
Cells, Cultured
DNA Damage*
DNA Repair*
Time Factors
Ultraviolet Rays
Xeroderma Pigmentosum / genetics*
Grant Support

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