Document Detail

Induction and repair of (6-4) photoproducts in normal human and xeroderma pigmentosum variant cells during the cell cycle.
MedLine Citation:
PMID:  7491119     Owner:  NLM     Status:  MEDLINE    
The reduced rate of (6-4) photoproduct repair observed in some cell lines may represent a more severe repair deficiency in some cohort of the cell cycle, such as S-phase. Radioimmunoassay was used to determine the kinetics of (6-4) photoproduct repair in normal human fibroblasts and xeroderma pigmentosum variant cells fractionated into different phases of the cell cycle by counterflow centrifugal elutriation. Ultraviolet fluence response curves indicated that the same amount of (6-4) photoproduct damage was induced at all phases of the cell cycle. The extent of (6-4) photoproduct repair in asynchronous XP variant cells was significantly reduced compared to normal human cells. However, the rate and extent of (6-4) photoproduct repair was constant throughout the cell cycle in both normal and XP variant cells. Hence, the UV hypersensitive and hypermutable phenotypes observed in XP variant cells are not attributable to cell cycle-dependent deficiencies in excision repair nor the yield of photodamage through the cell cycle.
D L Mitchell; J E Cleaver; M P Lowery; R R Hewitt
Related Documents :
11896679 - Pesticide roundup provokes cell division dysfunction at the level of cdk1/cyclin b acti...
20514219 - Perspectives in cell cycle regulation: lessons from an anoxic vertebrate.
21523509 - G1 cell cycle arrest signaling in hepatic injury after intraperitoneal sepsis in rats.
9502299 - Decrease in tumor cell contamination and progenitor cell yield in leukapheresis product...
15369799 - Rapamycin causes activation of protein phosphatase-2a1 and nuclear translocation of pcn...
6270139 - Phospholipid accumulation during the cell cycle in synchronous cultures of the yeast, s...
8946649 - Perfluorocarbon protects lung epithelial cells from neutrophil-mediated injury in an in...
24269809 - βtrcp-mediated ubiquitylation regulates protein stability of mis18β in a cell cycle-d...
19034449 - Stable xiap knockdown clones of hct116 colon cancer cells are more sensitive to trail, ...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Mutation research     Volume:  337     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-01-02     Completed Date:  1996-01-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  161-7     Citation Subset:  IM    
University of Texas M.D. Anderson Cancer Center, Smithville 78957, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Cycle / genetics*
Cell Separation
Cells, Cultured
DNA Damage*
DNA Repair*
Time Factors
Ultraviolet Rays
Xeroderma Pigmentosum / genetics*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Alternatively spliced human type 1 angiotensin II receptor mRNAs are translated at different efficie...
Next Document:  The yeast RAD2, but not RAD1, gene is involved in the transcription-coupled repair of thymine glycol...