| Induction of reactive oxygen species in bovine neutrophils is CD11b, but not dectin-1-dependent. | |
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MedLine Citation:
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PMID: 21106254 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Neutrophils play a key role in initiating an innate immune response, being the first type of immune cell arriving at the site of injury or infection. These cells are able to mount a direct anti-bactericidal response by the production of reactive oxygen or reactive nitrogen species (ROS/RNS). An important component of the host innate immune response is recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs). Toll-like receptors (TLRs) are an important family of PRRs and, are a key component in activation of innate immune mechanisms. In the present study we described the presence of mRNA transcripts for TLR1, TLR2, TLR4, TLR6, TLR7 and TLR10 in bovine neutrophils. In contrast, the presence of mRNA transcripts for TLR3 varied between animals, whereas no transcripts were detected for TLR5, TLR8, TLR9 or the C-type lectin receptor dectin-1 in neutrophils isolated from bovine blood. Additionally, zymosan, a dectin-1/TLR2 ligand, induced ROS, but not RNS production in a CD11b-, but not dectin-1-dependent manner. This effect was dependent on Store Operated Calcium Entry (SOCE), and partially inhibited using monoclonal antibodies to CD11b. Taken together, our data describe the presence of specific PRRs transcripts in the mRNA isolated from bovine neutrophil and show a CD11b-/Ca(2+) dependent ROS production by these cells. |
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Authors:
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I Conejeros; R Patterson; R A Burgos; C Hermosilla; D Werling |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-30 |
Journal Detail:
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Title: Veterinary immunology and immunopathology Volume: 139 ISSN: 1873-2534 ISO Abbreviation: Vet. Immunol. Immunopathol. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8002006 Medline TA: Vet Immunol Immunopathol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 308-12 Citation Subset: IM |
Copyright Information:
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Crown Copyright © 2010. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Laboratory of Inflammation Pharmacology, Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Science, Universidad Austral de Chile, P.O. Box 567, Valdivia, Chile. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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