Document Detail

Induction of prostaglandin E synthase by gastroesophageal reflux contents in normal esophageal epithelial cells and esophageal cancer cells.
MedLine Citation:
PMID:  17439595     Owner:  NLM     Status:  MEDLINE    
The synthesis of prostaglandin E2 (PGE2) requires cyclooxygenase (COX) and prostaglandin E synthase (PGES). There are two forms of PGES: cytosolic PGES (cPGES) and microsomal PGES (mPGES)-1. In this study, we investigated the effects of gastroesophageal reflux (GER) contents on PGES and COX-2 in esophageal cells. We incubated a human normal esophageal cell line, two esophageal squamous cell carcinoma (SCC) cell lines, and two esophageal adenocarcinoma (ADC) cell lines with GER contents. The production of PGE2 by these cells was assayed with an enzyme immunoassay kit. The protein expression of COX-2, cPGES, and mPGES-1 was confirmed by immunoblot analysis. The following results were obtained: GER contents induced the expression of COX-2 in all five cell lines. In normal esophageal cells, cPGES, but not mPGES-1, was detected in the cytosolic fraction. GER contents induced the expression of cPGES in the microsomal fraction. In SCC cells, cPGES was expressed in the cytosolic fraction, and mPGES-1 was expressed in the microsomal fraction. GER contents induced the expression of mPGES-1 in the microsomal fraction. In ADC cells, cPGES was expressed in both the cytosolic and microsomal fractions. GER contents induced the expression of both cPGES and mPGES-1 in the microsomal fraction. In conclusion, our results suggest that GER contents induce PGE2 production in esophageal cells. However, there are different isoforms of PGES in normal cells, SCC cells, and ADC cells.
T Soma; Y Shimada; A Kawabe; J Kaganoi; K Kondo; M Imamura; S Uemoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus / I.S.D.E     Volume:  20     ISSN:  1120-8694     ISO Abbreviation:  Dis. Esophagus     Publication Date:  2007  
Date Detail:
Created Date:  2007-04-18     Completed Date:  2007-07-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809160     Medline TA:  Dis Esophagus     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  123-9     Citation Subset:  IM    
Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
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MeSH Terms
Adenocarcinoma / metabolism*,  pathology
Carcinoma, Squamous Cell / metabolism*,  pathology
Cell Line
Cyclooxygenase 2 / metabolism
Esophageal Neoplasms / metabolism*,  pathology
Esophagus / cytology,  metabolism*
Gastroesophageal Reflux / metabolism*
Intramolecular Oxidoreductases / biosynthesis*
Membrane Proteins / metabolism
Reg. No./Substance:
0/Membrane Proteins; EC 2; EC protein, human; EC 5.3.-/Intramolecular Oxidoreductases; EC synthase

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