Document Detail


Induction of programmed cell death in a dorsal root ganglia X neuroblastoma cell line.
MedLine Citation:
PMID:  8515249     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Growth factor-dependent neurons die when they are deprived of their specific growth factor. This "programmed" cell death (PCD) requires macromolecular synthesis and is distinct from necrotic cell death. To investigate the mechanisms involved in neuronal PCD, we have studied the sequence of events that occur when a neuronal cell line (F-11: mouse neuroblastoma X rat dorsal root ganglia) is deprived of serum in a manner analogous to growth factor deprivation from neurons. Protein synthesis was inhibited within the first 8 h of serum deprivation, while DNA cleavage into nucleosome ladders was prominent by 24 h. The DNA cleavage could be inhibited by cycloheximide, consistent with a requirement for protein synthesis. In contrast, mitochondrial function was not compromised by serum deprivation. Rather, the cells appeared to be metabolically activated after serum removal as shown by an increased reduction of MTT by mitochondrial dehydrogenases and an increase in cellular autofluorescence, which is thought to be due to elevated levels of NADH and flavoproteins. Assessment of cell viability by propidium iodide staining showed no indication of cell death within 24 h. After 48 h of serum deprivation, cells decreased in size and increased propidium iodide uptake. Thus, serum deprivation activates PCD in F-11 cells and may be a useful model to study the intracellular events responsible for PCD.
Authors:
M D Linnik; M D Hatfield; M D Swope; N K Ahmed
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neurobiology     Volume:  24     ISSN:  0022-3034     ISO Abbreviation:  J. Neurobiol.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-07-19     Completed Date:  1993-07-19     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0213640     Medline TA:  J Neurobiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  433-46     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Marion Merrell Dow Research Institute, Cincinnati, Ohio 45215-6300.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / physiology*
Cell Survival / physiology
DNA / metabolism
Flow Cytometry
Ganglia, Spinal / cytology*
Hybrid Cells / physiology*
Metallothionein / metabolism
Methionine / metabolism
Mice
Neuroblastoma / pathology*
Oxidation-Reduction
Rats
Sulfur Radioisotopes / diagnostic use
Chemical
Reg. No./Substance:
0/Sulfur Radioisotopes; 63-68-3/Methionine; 9007-49-2/DNA; 9038-94-2/Metallothionein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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