| Induction and potentiation of diterpenoid tanshinone accumulation in Salvia miltiorrhiza hairy roots by beta-aminobutyric acid. | |
| | |
MedLine Citation:
|
PMID: 15672269 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The non-protein amino acid beta-aminobutyric acid (BABA) is a proven inducer of plant defense against pathogens. This work examines its effect on the production of diterpenoid tanshinones in Salvia miltiorrhiza hairy root cultures, both separately and in combination with a yeast elicitor (YE, the carbohydrate fraction of yeast extract). In the absence of YE, BABA at 0.1, 1 and 2 mM caused a dose-dependent enhancement of tanshinone accumulation, with up to a 4.5-fold increase (from 0.24 to 1.09 mg/g DW) in total content of three major tanshinones (cryptotanshinone, tanshinone I and tanshinone IIA) in the hairy roots. The combination of BABA with YE treatment further enhanced tanshinone production, but only when the BABA treatment was applied to the culture a few days before the YE treatment. Compared with methyl jasmonate, BABA was more effective in enhancing tanshinone production. A 3-day pretreatment with 1 mM BABA followed by YE-treatment, increased the total tanshinone content of roots by 9.4 times to 2.26 mg/g cells, and the volumetric tanshinone yield of culture by 6.3 times (from 3.2 to 20.1 mg/l). The results suggest that BABA can strongly potentiate elicitor-induced secondary metabolism in plant tissue cultures. |
| | |
Authors:
|
Xiuchun Ge; Jianyong Wu |
Related Documents
:
|
19401169 - The effects of chronic repetitive transcranial magnetic stimulation on glutamate and ga... 9504989 - Sasp (small, acid-soluble spore proteins) and spore properties in bacillus thuringiensi... 6145629 - Evidence for a role of neurotransmitters in the mechanism of topical convulsant models. 21475879 - The bone anabolic carotenoids p-hydroxycinnamic acid and β-cryptoxanthin antagonize nf... 12039969 - Enzymatic properties of human cytosolic phospholipase a(2)gamma. 9707349 - Pharmacokinetic data of gamma-linolenic acid in healthy volunteers after the administra... 16660539 - 2,4-dichlorophenoxyacetic acid inhibits the outer membrane nadh dehydrogenase of plant ... 7435569 - Metabolism in man of 7-ketolithocholic acid: precursor of cheno- and ursodeoxycholic ac... 9811809 - Polyazamacrolides from ladybird beetles: ring-size selective oligomerization. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-01-26 |
Journal Detail:
|
Title: Applied microbiology and biotechnology Volume: 68 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2005 Aug |
Date Detail:
|
Created Date: 2005-07-28 Completed Date: 2005-10-20 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
|
Languages: eng Pagination: 183-8 Citation Subset: IM |
Affiliation:
|
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aminobutyric Acids
/
pharmacology* Diterpenes / metabolism* Dose-Response Relationship, Drug Phenanthrenes / metabolism* Plant Roots / metabolism Salvia miltiorrhiza / metabolism* Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Aminobutyric Acids; 0/Diterpenes; 0/Phenanthrenes; 35825-57-1/cryptotanshinone; 541-48-0/3-aminobutyric acid; 568-73-0/tanshinone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Production of organic acids by Corynebacterium glutamicum under oxygen deprivation.
Next Document: Biodegradation of xenobiotics by anaerobic bacteria.