| Induction of platelet-derived growth factor B/c-sis by the v-erbA oncogene in glial cells. | |
| | |
MedLine Citation:
|
PMID: 7700635 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The v-erbA oncogene codes for a mutated form of the thyroid hormone receptor TR/c-erbA-alpha. Thyroid hormone (triiodothyronine, T3) regulates glial functions such as myelination and both astrocytes and oligodendrocytes have been shown to express thyroid hormone receptors (TRs). To study putative effects of v-erbA on glial precursors, we have expressed it in a glial clonal cell line established from early embryonal mouse brain. We have found that v-erbA increases cell survival in serum-free conditions. Moreover, v-erbA-expressing cells show a substantial growth in the presence of insulin or IGF-I, whereas normal and TR/c-erbA-over-expressing cells progressively degenerate. By Northern blotting, immunofluorescence, immunoprecipitation, and neutralization experiments, we show that v-erbA actions are mediated by an increase in the levels of PDGF B/c-sis mRNA and protein. We used anti-PDGF receptor and anti-phosphotyrosine antibodies to show the constitutive activation of PDGF receptors in B3.1 + v-erbA cells, and neutralizing anti-PDGF antibodies to demonstrate that v-erbA enhances the secretion of active PDGF into the culture medium. Our data indicate that v-erbA induces PDGF B/c-sis, a factor involved in the generation of gliomas, the most common central nervous system tumor in humans. |
| | |
Authors:
|
T Iglesias; S Llanos; M López-Barahona; B Seliger; A Rodríguez-Peña; J Bernal; A Muñoz |
Related Documents
:
|
3118365 - Thyroid hormones regulate levels of thyrotropin-releasing-hormone mrna in the paraventr... 14759075 - Localization and expression of thyroid hormone receptors normal and neoplastic human th... 7843995 - Hybridocytochemical and immuno-ultrastructural study of calcitonin gene expression in c... 9777755 - "spot 14" protein: a metabolic integrator in normal and neoplastic cells. 10910765 - Socs: physiological suppressors of cytokine signaling. 17097285 - Celecoxib enhances doxorubicin-induced cytotoxicity in mda-mb231 cells by nf-kappab-med... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Oncogene Volume: 10 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 1995 Mar |
Date Detail:
|
Created Date: 1995-05-03 Completed Date: 1995-05-03 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8711562 Medline TA: Oncogene Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 1103-10 Citation Subset: IM |
Affiliation:
|
Instituto de Investigaciones Biomédicas, C.S.I.C., Madrid, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
3T3 Cells Animals Cell Division / genetics Cell Line Cell Survival / genetics Genes, erbA* Mice Neuroglia / cytology, metabolism* Platelet-Derived Growth Factor / biosynthesis*, genetics Proto-Oncogene Proteins / biosynthesis*, genetics Proto-Oncogene Proteins c-sis RNA, Messenger / genetics, metabolism Receptors, Thyroid Hormone / physiology |
| Chemical | |
Reg. No./Substance:
|
0/Platelet-Derived Growth Factor; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-sis; 0/RNA, Messenger; 0/Receptors, Thyroid Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A reciprocal translocation (1;15) (36.2;q24) in a neuroblastoma cell line is accompanied by DNA dupl...
Next Document: Expression of an amphibian homolog of the Eph family of receptor tyrosine kinases is developmentally...