Document Detail


Induction of permeability changes and death of vertebrate cells is modulated by the virulence of Entamoeba spp. isolates.
MedLine Citation:
PMID:  12798929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although Entamoeba histolytica is capable of inducing an apoptotic response in vertebrate cells in vitro (Cell. Microbiol. 2 (2000) 617), it is not known whether vertebrate cell death requires direct amoeba-vertebrate cell contact or simply the presence of amoebae in the area of the vertebrate cells. In addition, Entamoeba spp. vary in their virulence and pathogenicity. The potential effects of these critical parameters also have not been elucidated. We tested the virulent HM-1:IMSS isolate and the non-virulent Rahman isolate of E. histolytica, and the non-virulent E. dispar CYNO16:TPC isolate against two vertebrate cell lines, HeLa and Chinese hamster ovary cells in vitro using ethidium homodimer as a fluorescent indicator of changes in vertebrate cell permeability. Fluorescence appeared in vertebrate cell nuclei within approximately 2-3 min of contact between HM-1 amoebae and vertebrate cells independent of vertebrate cell type. However, vertebrate cells in the immediate vicinity of but not contacted by HM-1 amoebae were not affected. In contrast, although both E. histolytica Rahman and E. dispar CYNO16 amoebae moved freely among and contacted vertebrate cells, the nuclei of the vertebrate cells never fluoresced implying that the cells remained alive and impermeant to the ethidium homodimer. This is the first demonstration that direct contact between virulent amoebae and vertebrate cells is required to kill vertebrate cells and that the process is restricted to virulent Entamoeba isolates. An understanding at the molecular level of the processes involved could help to reduce the pathology associated with this parasite.
Authors:
James A Dvorak; Seiki Kobayashi; Tomoyoshi Nozaki; Tsutomu Takeuchi; Chizuko Matsubara
Related Documents :
22361669 - Autophagy prolongs survival after nfκb inhibition in b-cell lymphomas.
9623919 - Human immunodeficiency virus type 1 (hiv-1) infection and transcytosis activity of a hi...
22466439 - A new cell-based reporter system for sensitive screening of nuclear export inhibitors.
16214359 - Steroidal saponins from calamus insignis, and their cell growth and cell cycle inhibito...
7803489 - The metabolism of vitamin a to 3,4-didehydroretinol can be demonstrated in human kerati...
11497289 - The carcinostatic and proapoptotic potential of 4-hydroxynonenal in hela cells is assoc...
11372069 - A new artificial nerve graft containing rolled schwann cell monolayers.
9243279 - Origin of the central cells of erythroblastic islands in fetal mouse liver: ultrahistoc...
20102369 - Antiproliferative activity of purine nucleoside phosphorylase multisubstrate analogue i...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Parasitology international     Volume:  52     ISSN:  1383-5769     ISO Abbreviation:  Parasitol. Int.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-06-11     Completed Date:  2003-09-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9708549     Medline TA:  Parasitol Int     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  169-73     Citation Subset:  IM    
Affiliation:
Laboratory of Malaria and Vector Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0425, USA. jdvorak@niaid.nih.gov
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cell Death / physiology
Cells, Cultured
Cricetinae
Entamoeba / classification,  isolation & purification,  pathogenicity*
Entamoeba histolytica / isolation & purification,  pathogenicity
Hela Cells
Humans
Permeability
Virulence

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Scuticociliata infection in the weedy sea dragon Phyllopteryx taeniolatus.
Next Document:  Use of a recombinant Clonorchis sinensis pore-forming peptide, clonorin, for serological diagnosis o...