| Induction of peptidylglycine alpha-amidating monooxygenase in N(18)TG(2) cells: a model for studying oleamide biosynthesis. | |
| | |
MedLine Citation:
|
PMID: 10631094 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The fatty-acid primary amide, oleamide, is a novel signaling molecule whose mechanism of biosynthesis is unknown. Recently, the N(18)TG(2) cell line was shown to synthesize oleamide from oleic acid, thereby demonstrating that these cells contain the necessary catalytic activities for generating the fatty-acid primary amide. The ability of peptide alpha-amidating enzyme, peptidylglycine-alpha-amidating monooxygenase (PAM; EC 1.14.17.3), to catalyze the formation of oleamide from oleoylglycine in vitro suggests this as a function for the enzyme in vivo. This investigation shows that N(18)TG(2) cells, in fact, express PAM and that cellular differentiation dramatically increases this expression. PAM expression was confirmed by the detection of PAM mRNA, PAM protein, and enzymatic activity that exhibits the functional characteristics of PAM isolated from mammalian neuroendocrine tissues. The regulated expression of PAM in N(18)TG(2) cells is consistent with the proposed role of PAM in the biosynthesis of fatty-acid primary amides and further establishes this cell line as a model for studying the pathway. |
| | |
Authors:
|
K J Ritenour-Rodgers; W J Driscoll; K A Merkler; D J Merkler; G P Mueller |
Related Documents
:
|
12006564 - Up-regulation of cyclooxygenase-2 expression and prostaglandin synthesis in endometrial... 11289104 - Promoter methylation regulates helicobacter pylori-stimulated cyclooxygenase-2 expressi... 8127004 - Interleukin-1-induced cyclooxygenase 2 expression is suppressed by cyclosporin a in rat... 11103814 - Differential effects of theaflavin monogallates on cell growth, apoptosis, and cox-2 ge... 9804904 - Effects of halothane, ketamine and nitrous oxide on dynorphin mrna expression in dorsal... 23602984 - Cannabinoids increase type 1 cannabinoid receptor expression in a cell culture model of... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 267 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2000 Jan |
Date Detail:
|
Created Date: 2000-02-23 Completed Date: 2000-02-23 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 521-6 Citation Subset: IM |
Copyright Information:
|
Copyright 2000 Academic Press. |
Affiliation:
|
Department of Chemistry and Biochemistry, Duquesne University, Pittsburgh, Pennsylvania 15282, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Base Sequence Cell Differentiation DNA Primers / genetics Enzyme Induction Mice Mixed Function Oxygenases / biosynthesis*, genetics Models, Biological Multienzyme Complexes* Neuroblastoma / enzymology, genetics, pathology Oleic Acids / biosynthesis* RNA, Messenger / genetics, metabolism RNA, Neoplasm / genetics, metabolism Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
|
NS34173/NS/NINDS NIH HHS; R15-GM59050/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/DNA Primers; 0/Multienzyme Complexes; 0/Oleic Acids; 0/RNA, Messenger; 0/RNA, Neoplasm; 301-02-0/oleylamide; EC 1.-/Mixed Function Oxygenases; EC 1.14.17.3/peptidylglycine monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interleukin-4 mediates STAT6 activation in 3T3-L1 preadipocytes but not adipocytes.
Next Document: Inhibition of food intake by central injection of anti-orexin antibody in fasted rats.