Document Detail


Induction of oxidative stress by high hydrostatic pressure in Escherichia coli.
MedLine Citation:
PMID:  15870304     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using leaderless alkaline phosphatase as a probe, it was demonstrated that pressure treatment induces endogenous intracellular oxidative stress in Escherichia coli MG1655. In stationary-phase cells, this oxidative stress increased with the applied pressure at least up to 400 MPa, which is well beyond the pressure at which the cells started to become inactivated (200 MPa). In exponential-phase cells, in contrast, oxidative stress increased with pressure treatment up to 150 MPa and then decreased again, together with the cell counts. Anaerobic incubation after pressure treatment significantly supported the recovery of MG1655, while mutants with increased intrinsic sensitivity toward oxidative stress (katE, katF, oxyR, sodAB, and soxS) were found to be more pressure sensitive than wild-type MG1655. Furthermore, mild pressure treatment strongly sensitized E. coli toward t-butylhydroperoxide and the superoxide generator plumbagin. Finally, previously described pressure-resistant mutants of E. coli MG1655 displayed enhanced resistance toward plumbagin. In one of these mutants, the induction of endogenous oxidative stress upon high hydrostatic pressure treatment was also investigated and found to be much lower than in MG1655. These results suggest that, at least under some conditions, the inactivation of E. coli by high hydrostatic pressure treatment is the consequence of a suicide mechanism involving the induction of an endogenous oxidative burst.
Authors:
Abram Aertsen; Philipp De Spiegeleer; Kristof Vanoirbeek; Maria Lavilla; Chris W Michiels
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  71     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-04     Completed Date:  2005-06-15     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2226-31     Citation Subset:  IM    
Affiliation:
Laboratory of Food Microbiology, Katholieke Universiteit Leuven, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Cytoplasm / metabolism
Escherichia coli / metabolism*
Hydrostatic Pressure*
Oxidative Stress*
Comments/Corrections

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