| Induction of ouabain-resistance mutation and cycle-dependent transformation of C3H/10T1/2 cells by N-nitroso-2-acetylaminofluorene. | |
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MedLine Citation:
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PMID: 2342496 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ouabain-resistance mutation and cell cycle-dependent transformation were studied concurrently in the C3H/10T1/2 cell line treated with N-nitroso-2-acetylaminofluorene (N-NO-AAF) or N-nitroso-N-2-fluorenylacetamide. N-NO-AAF is a new direct-acting mutagen that exhibits a very short half-life (34 min) in complete medium independent of cell number seeded. With 0.1-0.3 mM of N-NO-AAF, cytotoxicity was noted after exposure for 2 h, but another phase of cytotoxicity was observed between 8 and 24 h. N-NO-AAF was more toxic than its parent compound 2-AAF. Moreover, maximal mutation frequency at the Na+/K(+)-ATPase gene locus (ouar mutation) was attained within 30 or 40 min of exposure, dependent on dosage of N-NO-AAF. With 2-AAF, 2-AF and 2-nitrofluorene, however, no detectable mutants were found under the same conditions. In cell cycle-dependent transformation assays, cells were synchronized by release from confluence-induced arrest of proliferation, 2 concentrations of N-NO-AAF were added for 2 h at various intervals during the cell cycle. The results clearly revealed that cells in 2 specific time intervals were susceptible to malignant transformation, i.e., at 10 and 18 h (early S phase) after release from the block. |
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Authors:
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J K Lin; M L Kuo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Mutation research Volume: 230 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1990 May |
Date Detail:
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Created Date: 1990-06-27 Completed Date: 1990-06-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 35-43 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2-Acetylaminofluorene
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toxicity Animals Cell Cycle / drug effects* Cell Division / drug effects Cell Line Cell Survival / drug effects Cell Transformation, Neoplastic / chemically induced* Dose-Response Relationship, Drug Drug Resistance Half-Life Mutagens* Mutation Nitroso Compounds / toxicity* Ouabain / pharmacology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Mutagens; 0/Nitroso Compounds; 114119-92-5/N-nitroso-N(2)-fluorenylacetamide; 53-96-3/2-Acetylaminofluorene; 630-60-4/Ouabain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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