| Induction and nuclear accumulation of fos and jun proto-oncogenes in hypoxic cardiac myocytes. | |
| | |
MedLine Citation:
|
PMID: 8344964 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Hypoxic and ischemic stresses cause a series of well documented changes in myocardial cells and tissues, including increased anaerobic glycolysis, loss of contractility, changes in lipid and fatty acid metabolism, and eventual irreversible membrane damage and cell death. In this article we describe changes in the expression and regulation of the proto-oncogenes fos and jun in cardiac myocytes exposed to severe hypoxia. The mRNAs encoding c-Fos, c-Jun, Jun-D, and Jun-B were induced within 1 h of exposure to hypoxia, increased 5-10-fold between 1 and 4 h and then declined. These inductions coincided with loss in myocyte contractility but occurred before there was irreversible cell damage or significant ATP loss. Immunostaining with anti-Fos and anti-Jun antibodies revealed the accumulation of these proteins in hypoxic cell nuclei. Pre-treatment of cells with protein kinase inhibitors significantly repressed the response at the mRNA level. We propose that hypoxic stress in these cells activates signal transduction pathways, possibly involving protein kinases, that result in the inductions of fos and jun gene families. Therefore AP1 may regulate myocardial adaptive responses to hypoxia in advance of energy depletion, cell damage, or reoxygenation. |
| | |
Authors:
|
K A Webster; D J Discher; N H Bishopric |
Related Documents
:
|
15346654 - Nadph oxidase-mediated generation of reactive oxygen species is critically required for... 17159204 - Interference of chlorate and chlorite with nitrate reduction in resting cells of paraco... 8506994 - Pulmonary neuroendocrine cells in species at high altitude. 6252274 - Macrophage variants in oxygen metabolism. 22670904 - Inhibiting the hedgehog pathway in patients with the basal-cell nevus syndrome. 10719334 - Choice of anticoagulant can influence the analysis using fluorescence in situ hybridiza... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 268 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1993 Aug |
Date Detail:
|
Created Date: 1993-09-07 Completed Date: 1993-09-07 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 16852-8 Citation Subset: IM |
Affiliation:
|
Department of Cell and Molecular Biology, SRI International, Menlo Park, California 94025. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn Blotting, Northern Cell Hypoxia Cells, Cultured Myocardium / cytology, metabolism* Protein Kinase C / metabolism Proto-Oncogene Proteins c-fos / genetics*, metabolism Proto-Oncogene Proteins c-jun / genetics*, metabolism Proto-Oncogenes* Rats |
| Grant Support | |
ID/Acronym/Agency:
|
HL44578/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Partial assembly of the yeast vacuolar H(+)-ATPase in mutants lacking one subunit of the enzyme.
Next Document: Normal insulin receptor substrate-1 phosphorylation in autophosphorylation-defective truncated insul...