Document Detail


Induction of mucosal anti-HIV antibodies by facilitated transfection of airway epithelium with lipospermine/DNA complexes.
MedLine Citation:
PMID:  9373349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Expression of microbial protein sequences in eukaryotic cells transfected by transcriptional/translational permissive cDNA constructs can induce systemic humoral and cellular responses in vivo. Two methods of in vivo transfection have been described to date. One method uses large quantities of naked DNA injected into skeletal muscle. The second method uses relatively small quantities of DNA complexed to gold particles for bollistic penetration of the plasma membrane of keratinocytes. The major disadvantage of the bolistic method is that instrumentation is required which is not generally available. OBJECTIVES: The objectives of this study were to determine whether the use of DNA complexed with a cationic lipopolyamine could reduce the quantity of DNA required to induce systemic humoral responses following muscle transfection and whether similar DNA/lipopolyamine complexes could induce mucosal humoral responses following airway exposure. STUDY DESIGN: Balb/c mice were exposed by nasal aerosol or intramuscular inoculation to a mammalian transcriptional/translational permissive DNA construct containing the entire sequence for the HIV-1 envelope polyprotein. Experimental animals were further segregated by the number of exposures at 3-week intervals and whether the DNA was complexed to dioctadecylamidoglycylspermine (DOGS) at a 5:1 molar charge ratio of DOGS/DNA. RESULTS: DOGS facilitated in vivo transfection of mouse muscle reduced the quantity of DNA required for a systemic humoral response to surface expressed HIV-envelope proteins by one order of magnitude. Exposure of airway mucosa to both 10 micrograms and 1 microgram quantities of DNA complexed to DOGS produced systemic humoral responses to HIV-envelope as well as mucosal antibodies in pulmonary and colonic epithelia. Molecular modeling of DOGS/DNA complexes at the 5:1 charge ratio used in this study indicates that the DNA component is not exposed to aqueous solvents and may be relatively resistant to degradation under common biological environments. CONCLUSION: Facilitated transfer of DNA by DOGS to transcriptional/translational competent cells offers several distinct advantages to the use of DNA alone. Since significantly smaller amounts of DNA are required, the potential for the induction of antibodies against DNA itself lessens the likelihood for the development of a lupus-like syndrome. More importantly, however, is the apparent ability to transfect mucosal cells which results in the development of specific mucosal immune responses. This procedure may allow the development of general methods for the induction of mucosal immunity at the level of entry for mucosal pathogens without the disadvantages inherent in live attenuated vectors.
Authors:
W M Mitchell; S T Rosenbloom; J Gabriel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Immunotechnology : an international journal of immunological engineering     Volume:  1     ISSN:  1380-2933     ISO Abbreviation:  Immunotechnology     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1998-01-05     Completed Date:  1998-01-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9511979     Medline TA:  Immunotechnology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  211-9     Citation Subset:  IM; X    
Affiliation:
Department of Pathology, Vanderbilt University, Nashville, TN 37232, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bronchi / cytology*
DNA / administration & dosage*,  metabolism
Epithelial Cells / cytology*
Female
Gene Transfer Techniques
Glycine / administration & dosage,  analogs & derivatives*,  metabolism
HIV Antibodies / biosynthesis*
HIV Envelope Protein gp120 / immunology
HIV Envelope Protein gp160 / immunology
HIV Infections / immunology*
Immunity, Mucosal
Mice
Mice, Inbred BALB C
Models, Molecular
Spermine / administration & dosage,  analogs & derivatives*,  metabolism
Transfection / methods*
Grant Support
ID/Acronym/Agency:
AI31371/AI/NIAID NIH HHS; AI33815/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/HIV Antibodies; 0/HIV Envelope Protein gp120; 0/HIV Envelope Protein gp160; 124050-77-7/dioctadecylamidoglycylspermine; 56-40-6/Glycine; 71-44-3/Spermine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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