Document Detail

Induction of metallothionein by superantigenic bacterial exotoxin: probable involvement of the immune system.
MedLine Citation:
PMID:  8280785     Owner:  NLM     Status:  MEDLINE    
Metallothionein (MT) can be induced in mouse liver by a bacterial exotoxin, toxic shock syndrome toxin-1 (TSST-1). Hepatic MT was induced by TSST-1 in a dose-dependent manner from 100 micrograms/kg through 3 mg/kg in CF-1 mice, and by 6 h the induction was almost maximal. The increase of hepatic MT occurred at the mRNA level, also, and both MT-I and II mRNAs increased coordinately. Because TSST-1 is a superantigen, it was investigated whether TSST-1 induces MT through cytokines as a consequence of immunostimulation. In low-cytokine-producing mice (C3H/HeJ), up to a dose of 1 mg/kg of TSST-1, there was only 2- to 3-fold increase of hepatic MT. In contrast, in normal-cytokine-producing mice (C3Heb/FeJ), TSST-1 increased MT in a dose-dependent manner, and at a dose of 1 mg/kg, there was a 25-fold increase in hepatic MT. This suggests that activation of the immune system is probably involved in the induction of MT by TSST-1. Studies on the role of specific hepatic cytokines (IL-1, TNF-alpha, and IL-6) in TSST-mediated hepatic MT induction showed that TSST-1 did not increase hepatic IL-1 or TNF-alpha significantly over controls in any of the mouse strains studied. In contrast, TSST-1 induced hepatic IL-6 in all three strains of mice. However, in CF-1 and C3Heb/FeJ mice (normal-cytokine-producing) IL-6 induction preceded MT mRNA induction, but in C3H/HeJ mice (low-cytokine-producing), IL-6 induction did not precede MT mRNA induction.
S Choudhuri; J M McKim; C D Klaassen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1225     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-02-15     Completed Date:  1994-02-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  171-9     Citation Subset:  IM    
Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City 66160-7417.
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MeSH Terms
Antigens, Bacterial / pharmacology*
Bacterial Toxins*
Enterotoxins / pharmacology*
Interleukin-1 / analysis
Interleukin-6 / analysis
Liver / drug effects*,  metabolism
Metallothionein / genetics,  metabolism*
Mice, Inbred C3H
RNA, Messenger / analysis
Time Factors
Grant Support
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Toxins; 0/Enterotoxins; 0/Interleukin-1; 0/Interleukin-6; 0/RNA, Messenger; 0/Superantigens; 0/enterotoxin F, Staphylococcal; 9038-94-2/Metallothionein

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