Document Detail

Induction of melanogenesis by 4'-O-methylated flavonoids in B16F10 melanoma cells.
MedLine Citation:
PMID:  23208771     Owner:  NLM     Status:  Publisher    
Agents to control melanogenesis are in demand for the development of cosmetics to improve pigmentation disorders of skin and hair. In this study, we examined and evaluated the effects of flavonoids on melanogenesis in the melanogenic cells model, murine B16F10 melanoma cells. In the course of this study, we found that incubation of the cells in a medium containing 10 μM of the 4'-O-methylated flavonoids, diosmetin (4'-O-methylluteolin), acacetin (4'-O-methylapigenin) or kaempferide (4'-O-methylkaempferol), increased the melanin contents of the cells 3- to 7-fold higher than the control cells. The concentration-dependence test revealed that 20 μM acacetin showed the highest effect, up to 33-fold higher than the vehicle. On the other hand, the corresponding 4'-OH-type flavonoids, luteolin, apigenin and kaempferol, had a significantly smaller effect. Furthermore, by evaluating the melanogenic proteins, we found that the cells treated with 4'-O-methylated flavonoids showed higher tyrosinase activity, as well as upregulation of tyrosinase expression, preceded by activation of cAMP response element binding protein (CREB) and extracellular signal-regulated kinases types 1 and 2 (ERK1/2). These results indicate that the 4'-O-methyl group of flavonoids plays an important role in the induction of melanogenesis by activating its major signal transduction pathway through the upregulation of phospho-CREB in murine B16F10 melanoma cells.
Ippei Horibe; Yudai Satoh; Yuki Shiota; Ayako Kumagai; Nanao Horike; Hiroshi Takemori; Shinichi Uesato; Shuji Sugie; Katsuyoshi Obata; Hidehisa Kawahara; Yasuo Nagaoka
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-4
Journal Detail:
Title:  Journal of natural medicines     Volume:  -     ISSN:  1861-0293     ISO Abbreviation:  J Nat Med     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101518405     Medline TA:  J Nat Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Nakano Seiyaku Co. Ltd, 6-20 Kitainoue-cho, Higashino, Yamashina-ku, Kyoto, 607-8141, Japan.
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