Document Detail


Induction of meiotic gynogenesis in Atlantic cod (Gadus morhua L.) through pressure shock.
MedLine Citation:
PMID:  21840654     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The Atlantic cod, Gadus morhua, is one of the most important species for commercial fisheries and a promising candidate for aquaculture. Precocious sexual maturation of males is one of the major issues compromising large scale production. The potential approaches to this problem include production of all female populations. Consequently, the objective of this study was to develop an effective protocol to induce meiotic gynogenesis in the Atlantic cod by using hydrostatic pressure shock. Our first experiment tested the relevance of gamete quality on achievement of chromosome manipulation and identified the best time interval between fertilization and pressure shock. Our second experiment was designed to determine the optimal pressure value and duration of the pressure shock. Eight combinations of pressure values and durations were tested. Among them, the 34.47MPa/6min combination gave the best survival rate (23.6±3.9%), the highest percentage of normal larvae (15.7±3.6%), and the highest percentage of meiotic diploids (88.89%). In both experiments, haploid controls served as an indirect reference for paternal DNA inactivation. Chromosome counting confirmed the restoration of diploidy in gynogenetic fish. The present study optimizes a procedure for the induction of meiotic gynogenesis in the Atlantic cod, thus laying the basis for further applications towards producing monosex and defining the sex determination system.
Authors:
Laura Ghigliotti; Sylvie L Bolla; Michal Duc; Oddvar H Ottesen; Igor Babiak
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-30
Journal Detail:
Title:  Animal reproduction science     Volume:  -     ISSN:  1873-2232     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-8-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Faculty of Biosciences and Aquaculture, University of Nordland, 8049 Bodø, Norway; Department of Biology, University of Genoa, Viale Benedetto XV 5, 16132 Genoa, Italy.
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