| Induction of medulloblastoma cell apoptosis by sulforaphane, a dietary anticarcinogen from Brassica vegetables. | |
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MedLine Citation:
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PMID: 14670615 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There is increasing evidence that a variety of natural substances derived from the diet may act as potent chemopreventive agents. In this work, we show that DAOY cells, a widely used model of metastatic medulloblastoma (MBL), are highly sensitive to sulforaphane, a naturally occurring isothiocyanate from Brassica vegetables. Sulforaphane induced DAOY cell death by apoptosis, as determined by DNA fragmentation and chromatin condensation. DAOY apoptosis correlates with the induction of caspase-3 and -9 activities, resulting in the cleavage of PARP and vimentin. Both the cytotoxic effect and apoptotic characteristics induced by sulforaphane were reversed by zVAD-fmk, a broad spectrum caspase inhibitor, demonstrating the important role of caspases in its cytotoxic effect. These results identify sulforaphane as a novel inducer of MBL cell apoptosis, supporting the potential clinical usefulness of diet-derived substances as chemopreventive agents. |
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Authors:
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Denis Gingras; Martin Gendron; Dominique Boivin; Albert Moghrabi; Yves Théorêt; Richard Béliveau |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer letters Volume: 203 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2003-12-12 Completed Date: 2004-02-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 35-43 Citation Subset: IM |
Affiliation:
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Centre de Cancérologie Charles-Bruneau, Hôpital Ste-Justine, 3175 Chemin Côte-Ste-Catherine, H3T 1C5 Montréal, Que., Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticarcinogenic Agents / pharmacology* Apoptosis / drug effects Caspase 3 Caspase 9 Caspases / metabolism Cattle DNA Fragmentation HL-60 Cells Humans Medulloblastoma / enzymology, prevention & control* Neoplasm Metastasis / prevention & control Proteins / metabolism Thiocyanates / pharmacology* Tumor Cells, Cultured Vegetables / chemistry* Vimentin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anticarcinogenic Agents; 0/Proteins; 0/Thiocyanates; 0/Vimentin; 4478-93-7/sulforafan; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/CASP9 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases |
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