Document Detail


Induction of mammary gland hyperplasia and carcinomas in transgenic mice expressing human cyclin E.
MedLine Citation:
PMID:  8972226     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Deregulated expression of several cell cycle regulatory genes has been demonstrated to be associated with cancer. In particular, a strong correlation has been established between inappropriate cyclin E expression and human breast cancer. To determine the ability of cyclin E to play a causative role in mammary tumorigenesis, regulatory sequences from the ovine beta-lactoglobulin gene were utilized to specifically target expression of human cyclin E to the mammary glands of pregnant and lactating mice. Lactating mammary glands of transgenic mice expressing cyclin E contained areas of hyperplasia, primarily papillary projections of hyperplastic cells, which were rarely observed in lactating glands of control mice. Over 10% of female cyclin E transgenic mice have developed mammary carcinomas, with latencies ranging from 8 to 13 months. Tumor analysis revealed the presence of transgene-specific cyclin E RNA and protein, as well as cyclin E- and cdk2-associated kinase activity, suggesting that cyclin E is likely a contributing component of tumorigenic progression in this model system.
Authors:
D M Bortner; M P Rosenberg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  17     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-01-22     Completed Date:  1997-01-22     Revised Date:  2012-06-25    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  453-9     Citation Subset:  IM    
Affiliation:
Department of Cellular Sciences, Glaxo Wellcome Research and Development, Research Triangle Park, North Carolina 27709, USA. bortner-dm@glaxo.com
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MeSH Terms
Descriptor/Qualifier:
Animals
CDC2-CDC28 Kinases*
Carcinoma / enzymology,  genetics*
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinases / metabolism
Cyclins / analysis,  genetics*,  physiology
Female
Gene Expression
Humans
Hyperplasia / genetics*
Lactation
Lactoglobulins / genetics
Lymphoma / enzymology
Mammary Glands, Animal / chemistry,  pathology*
Mammary Neoplasms, Experimental / enzymology,  genetics*
Mice
Mice, Transgenic
Pregnancy
Promoter Regions, Genetic / genetics
Protein Kinases / metabolism
Protein-Serine-Threonine Kinases / metabolism
Sheep
Chemical
Reg. No./Substance:
0/Cyclins; 0/Lactoglobulins; EC 2.7.-/Protein Kinases; EC 2.7.1.-/histone H1 kinase; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.22/CDC2-CDC28 Kinases; EC 2.7.11.22/CDK2 protein, human; EC 2.7.11.22/Cdk2 protein, mouse; EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/Cyclin-Dependent Kinases
Comments/Corrections

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