| Induction of macrophage migration by neurotoxic prion protein fragment. | |
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MedLine Citation:
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PMID: 19447501 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Prion diseases are characterized by accumulation of protease resistant isoforms of prion protein (PrP), and infiltration and activation of mononuclear phagocytes at the brain lesions. Interactions between prion proteins and immune cells during disease progression are still not very well understood. In the present study, multiwell chamber chemotaxis assay was carried out to assess the migratory response of macrophage cell line Ana-1 to a synthetic peptide homologous to residues 106-126 of the human prion protein. Specific protein kinase inhibitors were used to elucidate the signaling events underlying PrP106-126-induced macrophages migration, and a comparison with the signaling pattern of macrophage migration induced by substance P (SP) and N-formyl-methionyl-leucyl-phenylalanine (fMLP), respectively, was carried out. The results showed that PrP106-126 had a potent chemotactic effect on murine macrophage cell line Ana-1; that multiple signaling pathways might be involved in the PrP106-126-induced macrophage migrations; and that PrP106-126-induced chemotactic activity was similar to that induced by SP. These findings provide new insights into the mechanisms underlying the interaction between PrP and macrophages. |
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Authors:
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Haiyun Zhou; Xiangmei Zhou; Mohammed Kouadir; Zhongqiu Zhang; Xiaomin Yin; Lifeng Yang; Deming Zhao |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-14 |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 181 ISSN: 1872-678X ISO Abbreviation: J. Neurosci. Methods Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-06-15 Completed Date: 2009-08-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-5 Citation Subset: IM |
Affiliation:
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National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Count / methods Cell Line Cell Movement / drug effects* Chemotaxis / drug effects* Enzyme Inhibitors / pharmacology Macrophages / drug effects* Mice N-Formylmethionine Leucyl-Phenylalanine / pharmacology Peptide Fragments / pharmacology* Prions / pharmacology* Substance P / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Peptide Fragments; 0/Prions; 0/prion protein (106-126); 33507-63-0/Substance P; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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