Document Detail


Induction of large DNA palindrome formation in yeast: implications for gene amplification and genome stability in eukaryotes.
MedLine Citation:
PMID:  8978615     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Many amplified genes, including some oncogenes, are organized as large inverted repeats. How such giant palindromes are generated remains largely unknown. Recent studies of a palindrome in the ciliate Tetrahymena suggest a novel mechanism that requires chromosome breakage next to short inverted repeats. The prevalence of short inverted repeats in eukaryotic genomes raises the interesting possibility that this process may occur widely as a response to chromosome damage. Here we demonstrate that in Saccharomyces cerevisiae, large DNA palindromes are formed efficiently, probably by intramolecular recombination, when a double-strand break is introduced next to short inverted repeats. These results suggest a general mechanism for large palindromic DNA formation and reveal an important new source of genome instability resulting from chromosome breakage at selective sites.
Authors:
D K Butler; L E Yasuda; M C Yao
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  87     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-01-28     Completed Date:  1997-01-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1115-22     Citation Subset:  IM    
Affiliation:
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cell Differentiation / genetics
DNA, Fungal / genetics
DNA-Binding Proteins / genetics
Eukaryotic Cells / physiology
Fungal Proteins / genetics
Gene Amplification*
Genome
Molecular Sequence Data
Rad52 DNA Repair and Recombination Protein
Regulatory Sequences, Nucleic Acid*
Repetitive Sequences, Nucleic Acid / physiology*
Saccharomyces cerevisiae / cytology,  genetics*
Saccharomyces cerevisiae Proteins
Grant Support
ID/Acronym/Agency:
F32 GM13293/GM/NIGMS NIH HHS; F32 GM1434/GM/NIGMS NIH HHS; R01NIHGM26210/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/DNA-Binding Proteins; 0/Fungal Proteins; 0/RAD52 protein, S cerevisiae; 0/Rad52 DNA Repair and Recombination Protein; 0/Saccharomyces cerevisiae Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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