Document Detail


Induction of the lactose transport system in a lipid-synthesis-defective mutant of Escherichia coli.
MedLine Citation:
PMID:  4914567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to relate the biogenesis of the lactose transport system to lipid synthesis, a glycerol-requiring mutant of Escherichia coli K-12 with a specific defect in l-glycerol-3-phosphate synthesis was isolated and characterized. The defective enzyme is the biosynthetic l-glycerol-3-phosphate dehydrogenase [l-glycerol-3-phosphate: NAD (P) oxidoreductase, EC 1.1.1.8] which functions as a dihydroxyacetone phosphate reductase to provide l-glycerol-3-phosphate for lipid synthesis. In this mutant, removal of glycerol from the growth medium results in inhibition of the synthesis of protein, deoxyribonucleic acid, and phospholipid. Inhibition of phospholipid synthesis immediately follows glycerol removal, whereas the inhibition of deoxyribonucleic acid and protein synthesis is preceded by a short lag period. Glycerol starvation does not change the turnover pattern of previously synthesized phospholipids. The blocking of lipid synthesis by glycerol starvation causes a drastic decrease in inducibility of beta-galactoside transport activity relative to beta-galactosidase, indicating that induction of lactose transport requires de novo lipid synthesis.
Authors:
C C Hsu; C F Fox
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  103     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1970 Aug 
Date Detail:
Created Date:  1970-09-10     Completed Date:  1970-09-10     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  410-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / biosynthesis
Carbon Isotopes
DNA, Bacterial / biosynthesis
Escherichia coli / enzymology,  metabolism*
Galactosidases / metabolism
Glycerol / metabolism
Glycerolphosphate Dehydrogenase / metabolism
Isoleucine / metabolism
Lactose / metabolism*
Mutation
Operon
Phospholipids / biosynthesis*
Phosphorus Isotopes
Thymidine / metabolism
Tritium
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Carbon Isotopes; 0/DNA, Bacterial; 0/Phospholipids; 0/Phosphorus Isotopes; 10028-17-8/Tritium; 50-89-5/Thymidine; 56-81-5/Glycerol; 63-42-3/Lactose; 73-32-5/Isoleucine; EC 1.1.-/Glycerolphosphate Dehydrogenase; EC 3.2.1.-/Galactosidases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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