Document Detail


Induction of insulin resistance by high-sucrose feeding does not raise mean arterial blood pressure but impairs haemodynamic responses to insulin in rats.
MedLine Citation:
PMID:  12208775     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. This study was undertaken to further investigate the effects of a sucrose-enriched diet on vascular function and insulin sensitivity in rats. 2. Male Sprague-Dawley rats were randomized to receive a sucrose- or regular rat chow-diet for 4 weeks. A first group of sucrose- and chow-fed rats was instrumented with pulsed Doppler flow probes and intravascular catheters to determine blood pressure, heart rate, regional blood flows and insulin sensitivity in conscious rats. Insulin sensitivity was assessed by the euglycemic hyperinsulinemic clamp technique. Glucose transport activity was examined in isolated muscles by using the glucose analogue [(3)H]-2-deoxy-D-glucose. A second group of sucrose- and chow-fed rats was used to obtain information regarding nitric oxide synthase (NOS) isozymes protein expression in muscles, and determine endothelin content in vascular tissues isolated from both dietary groups. 3. Sucrose feeding was found to induce insulin resistance, but had no effect on resting blood pressure, heart rate, or regional haemodynamics. This insulin resistance was accompanied by alteration in the vascular responses to insulin. Insulin-mediated skeletal muscle vasodilation was impaired, whereas the mesenteric vasoconstrictor response was potentiated in sucrose-fed rats. A reduction in eNOS protein content in muscle and an increase in vascular endothelin peptide were noted in these animals. Moreover, a reduction in insulin-simulated glucose transport activity was also noted in muscles isolated from sucrose-fed rats. 4. Together these data suggest that a cluster of metabolic and haemodynamic abnormalities occur in response to the intake of simple sugars in rats.
Authors:
Marta Santuré; Maryse Pitre; André Marette; Yves Deshaies; Christian Lemieux; Richard Larivière; André Nadeau; Hélène Bachelard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of pharmacology     Volume:  137     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-04     Completed Date:  2003-02-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  185-96     Citation Subset:  IM    
Affiliation:
Hypertension Research Unit, Centre de Recherche du CHUL, CHUQ, Ste-Foy, (Quebec) Canada, G1V 4G2.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure / drug effects
Endothelin-1 / analysis
Hemodynamics / drug effects*
Insulin / pharmacology*
Insulin Resistance*
Male
Mesenteric Arteries / chemistry
Muscle, Skeletal / metabolism
Nitric Oxide Synthase / analysis
Rats
Rats, Sprague-Dawley
Sucrose / administration & dosage*
Chemical
Reg. No./Substance:
0/Endothelin-1; 11061-68-0/Insulin; 57-50-1/Sucrose; EC 1.14.13.39/Nitric Oxide Synthase
Comments/Corrections

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