| Induction of insulin-dependent diabetes mellitus by total pancreatectomy for pancreatic islet transplantation in cynomolgus monkeys. | |
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MedLine Citation:
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PMID: 22179578 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND/PURPOSE: In order to perform a preclinical trial of pancreatic islet transplantation (PIT) in nonhuman primates, insulin-dependent diabetes mellitus (IDDM) must be induced. Methods for IDDM induction are administration of streptozotocin (STZ) or total pancreatectomy (TP). While STZ-induced IDDM is not always reliable, TP is appropriate for IDDM induction. However, TP is very difficult because of the complex surgical procedure required, necessary confirmation of IDDM, and difficulty in postoperative management. In this study, we tried to establish a reliable IDDM model for PIT in cynomolgus monkeys. METHODS: TP was performed in 5 male cynomolgus monkeys (Macaca fascicularis). This was followed by scheduled measurements of blood glucose, C-peptide levels, insulin levels, oral intake, and insulin requirements. IDDM induction was confirmed by serum C-peptide levels and intravenous glucose tolerance test (IVGTT). Allogeneic PIT was performed 14 days later with immunosuppressive therapy. RESULTS: TP successfully induced IDDM in all animals, confirmed by reduction of fasting serum C-peptide levels. Negative responses of serum C-peptide levels and insulin in IVGTT provided further confirmation of IDDM induction. No mortality or morbidity was encountered in any of the animals. CONCLUSIONS: TP successfully induced IDDM for PIT in cynomolgus monkeys. |
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Authors:
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Tadashi Yoshida; Tomomi Suzuki; Masaaki Watanabe; Kenichiro Yamashita; Yasuyuki Koshizuka; Daisuke Kuraya; Masaomi Ogura; Hirofumi Kamachi; Michiaki Matsushita; Satoru Todo |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-17 |
Journal Detail:
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Title: Journal of hepato-biliary-pancreatic sciences Volume: - ISSN: 1868-6982 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101528587 Medline TA: J Hepatobiliary Pancreat Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of General Surgery, Hokkaido University Graduate School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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