Document Detail

Induction of increased levels of proteoglycan fragments in synovial fluid (SF) and increased levels of stromelysin in cartilage, synovium and SF by intraarticular injection of canine monocyte conditioned medium into dogs.
MedLine Citation:
PMID:  7799356     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To study the effects of the intraarticular injection of canine monocyte conditioned medium (cMCM) into dogs on proteoglycan fragment and stromelysin levels in the joint. METHODS: cMCM was injected intraarticularly into dogs, and the levels of proteoglycan fragments in synovial fluid (SF) as well as stromelysin levels in cartilage, synovium, and SF were assessed after 12 h. RESULTS: There was a 4-fold increase of proteoglycan fragment levels and a 6-fold increase in stromelysin levels in SF, and a 4.4-fold increase in stromelysin levels in cartilage extracts. Elevated mRNA levels were detected in both synovium and cartilage. By immunofluorescence staining, stromelysin was localized in chondrocytes throughout the cartilage and in synovial cells. CONCLUSION: Intraarticular injection of cMCM stimulated the expression of stromelysin mRNA and protein in cartilage and synovium and caused marked increases in stromelysin protein and proteoglycan fragment levels in SF.
M W Lark; K A MacNaul; S Donatelli; J McDonnell; L A Hoerrner; K A Stevens; C A Saphos; E K Bayne; N I Hutchinson; V L Moore
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of rheumatology     Volume:  21     ISSN:  0315-162X     ISO Abbreviation:  J. Rheumatol.     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1995-01-26     Completed Date:  1995-01-26     Revised Date:  2010-03-24    
Medline Journal Info:
Nlm Unique ID:  7501984     Medline TA:  J Rheumatol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  1716-24     Citation Subset:  IM    
Department of Immunology, Merck Research Laboratories, Rahway, NJ 07065.
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MeSH Terms
Arthritis / metabolism*
Cartilage, Articular / chemistry*,  pathology
Culture Media, Conditioned
Fluorescent Antibody Technique
Injections, Intra-Articular
Matrix Metalloproteinase 3
Metalloendopeptidases / analysis*,  biosynthesis,  genetics
Monocytes / physiology*
Peptide Fragments / analysis*
Proteoglycans / analysis*
RNA, Messenger / analysis,  genetics
Synovial Fluid / chemistry*
Synovial Membrane / chemistry*,  pathology
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Peptide Fragments; 0/Proteoglycans; 0/RNA, Messenger; EC 3.4.24.-/Metalloendopeptidases; EC Metalloproteinase 3

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