| Induction of in situ DNA double-strand breaks and apoptosis by 200 MeV protons and 10 MV X-rays in human tumour cell lines. | |
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MedLine Citation:
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PMID: 20954835 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To clarify the properties of clinical high-energy protons by comparing with clinical high-energy X-rays. MATERIALS AND METHODS: Human tumor cell lines, ONS76 and MOLT4, were irradiated with 200 MeV protons or 10 MV X-rays. In situ DNA double-strand breaks (DDSB) induction was evaluated by immunocytochemical staining of phosphorylated histone H2AX (γ-H2AX). Apoptosis was measured by flow-cytometry after staining with Annexin V. The relative biological effectiveness (RBE) was obtained by clonogenic survival assay. RESULTS: DDSB induction was significantly higher for protons than X-rays with average ratios of 1.28 (ONS76) and 1.59 (MOLT4) at 30 min after irradiation. However the differences became insignificant at 6 h. Also, apoptosis induction in MOLT4 cells was significantly higher for protons than X-rays with an average ratio of 2.13 at 12 h. However, the difference became insignificant at 20 h. RBE values of protons to X-rays at 10% survival were 1.06 ± 0.04 and 1.02 ± 0.15 for ONS76 and MOLT4, respectively. CONCLUSIONS: Cell inactivation may differ according to different timings and/or endpoints. Proton beams demonstrated higher cell inactivation than X-rays in the early phases. These data may facilitate the understanding of the biological properties of clinical proton beams. |
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Authors:
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Ariungerel Gerelchuluun; Zhengshan Hong; Lue Sun; Kenshi Suzuki; Toshiyuki Terunuma; Kiyoshi Yasuoka; Takeji Sakae; Takashi Moritake; Koji Tsuboi |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-18 |
Journal Detail:
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Title: International journal of radiation biology Volume: 87 ISSN: 1362-3095 ISO Abbreviation: Int. J. Radiat. Biol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-14 Completed Date: 2011-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8809243 Medline TA: Int J Radiat Biol Country: England |
Other Details:
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Languages: eng Pagination: 57-70 Citation Subset: IM; S |
Affiliation:
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Proton Medical Research Center, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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radiation effects* Cell Line, Tumor DNA Breaks, Double-Stranded* Dose-Response Relationship, Radiation Histones / metabolism Humans Medulloblastoma / metabolism, pathology, radiotherapy Particle Accelerators Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / metabolism, pathology, radiotherapy Protons* Radiotherapy, High-Energy / instrumentation Relative Biological Effectiveness Synchrotrons Tumor Stem Cell Assay |
| Chemical | |
Reg. No./Substance:
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0/H2AFX protein, human; 0/Histones; 0/Protons |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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