| Induction of human mammary-associated serum amyloid A3 expression by prolactin or lipopolysaccharide. | |
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MedLine Citation:
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PMID: 12589816 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In most mammalian species, serum amyloid A isoform 3 (SAA3) appears to be the predominant SAA isoform expressed extrahepatically. However, human SAA3 gene expression has not been detected previously and, therefore, this gene was referred to as a pseudogene. We report for the first time the transcriptional expression of human SAA3. Human SAA3 gene expression was detected by RT-PCR after stimulation of mammary gland epithelial cells with either prolactin (PRL) or lipopolysaccharide (LPS). The full-length 655bp cDNA sequence for this mammary-associated serum amyloid A3 (M-SAA3) was obtained using 5' and 3' rapid amplification of cDNA ends (RACE). The human M-SAA3 transcript would conceptually translate into a 42 residue mature protein, which is smaller than other mammalian SAA3 isoforms that are typically 104-113 amino acids in length. This study defines the cDNA sequence for human SAA3 and also demonstrates the upregulation of M-SAA3 expression in response to the lactational hormone PRL or to an acute phase stimulant such as LPS. |
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Authors:
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Marilynn A Larson; Shu H Wei; Annika Weber; Allen T Weber; Thomas L McDonald |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 301 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-02-18 Completed Date: 2003-04-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 1030-7 Citation Subset: IM |
Affiliation:
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Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AY209188 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Breast / drug effects*, metabolism* Cell Line DNA Primers / genetics DNA, Complementary / genetics Epithelial Cells / drug effects, metabolism Female Gene Expression / drug effects Humans Lipopolysaccharides / pharmacology* Molecular Sequence Data Prolactin / pharmacology* Protein Isoforms / genetics Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Serum Amyloid A Protein / genetics* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Complementary; 0/Lipopolysaccharides; 0/Protein Isoforms; 0/SAA3P protein, human; 0/Serum Amyloid A Protein; 9002-62-4/Prolactin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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