| Induction of human leukocyte interferon by heat-treated poly I: poly C. | |
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MedLine Citation:
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PMID: 6838554 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Synthetic double stranded RNA (poly I: poly C) was prepared from polyinosinic acid (poly I) and polycytidylic acid (poly C) by heat-treated followed by gradual cooling, and was used for induction of human leukocyte interferon (IFN). When poly I: poly C solution was heated at 37 - 65 degrees C for 30 minutes, high activity of human IFN (10,000 - 60,000 i. u./ml) was obtained. In this system, the optimum molecular weight of poly I: poly C to induce IFN was 12.6 - 17.6 S. The properties of induced IFN were heat- and acid- stable, and it was neutralized with anti-IFN alpha serum. So, it was confirmed that it was IFN alpha. |
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Authors:
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M Tamura; S Sasakawa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 110 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1983 Feb |
Date Detail:
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Created Date: 1983-05-27 Completed Date: 1983-05-27 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 851-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel Humans Interferon Type I / biosynthesis*, isolation & purification Kinetics Molecular Weight Neutrophils / drug effects, physiology* Poly I-C / pharmacology* Temperature |
| Chemical | |
Reg. No./Substance:
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0/Interferon Type I; 24939-03-5/Poly I-C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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