Document Detail


Induction of human lampbrush chromosomes.
MedLine Citation:
PMID:  23296495     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We previously demonstrated that sperm heads from amphibians (Xenopus and Rana) and zebrafish (Danio) could form giant lampbrush chromosomes when injected into the nucleus of amphibian oocytes. However, similar experiments with mammalian sperm heads were unsuccessful. Here, we describe a slightly modified procedure and demonstrate that human sperm heads can form giant lampbrush chromosomes when injected into the oocyte nucleus of the frog Xenopus laevis or the newt Notophthalmus viridescens. Human and other mammalian chromosomes do not form recognizable lampbrush chromosomes in their own oocytes or in any somatic cells. These experiments thus demonstrate that the lampbrush condition is an inducible state and that the amphibian oocyte nucleus contains all factors required to remodel the inactive chromatin of a mammalian sperm into a transcriptionally active state. They also demonstrate that absence of lampbrush chromosomes from human oocytes must relate to specific features of mammalian oogenesis, not to permanent genetic or epigenetic changes in the chromatin.
Authors:
Ji-Long Liu; Joseph G Gall
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  20     ISSN:  1573-6849     ISO Abbreviation:  Chromosome Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-02-06     Completed Date:  2013-07-26     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  971-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / genetics,  metabolism
Chromatin / genetics,  ultrastructure
Chromosomes, Human / genetics*,  ultrastructure*
Fluorescent Antibody Technique
Humans
Male
Oocytes / cytology,  metabolism
Oogenesis
Salamandridae / genetics
Sperm Head / metabolism,  ultrastructure
Temperature
Xenopus laevis / genetics
Zebrafish / genetics
Grant Support
ID/Acronym/Agency:
R01 GM033397/GM/NIGMS NIH HHS; R01 GM33397/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Chromatin
Comments/Corrections

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