Document Detail

Induction of heat shock protein in interdental cells by hyperthermia.
MedLine Citation:
PMID:  1470456     Owner:  NLM     Status:  MEDLINE    
The effect of hyperthermia on induction of the 72 kilodalton (kDa) heat shock protein (HSP72) was examined in interdental cells of the guinea pig cochlea. After being immersed in a water bath of either normal body temperature (37 degrees C, control condition) or 43 degrees C (hyperthermic condition), animals were killed either 0, 1, 2, 6, or 18 hours later. Cochlear sections were incubated with a monoclonal antibody raised against HSP72 and relative staining densities were quantified with a light microscopic image analysis system. Optical densities of the interdental cell region of animals receiving hyperthermia treatment were significantly greater than those of animals in the control group. Further analysis revealed that levels of HSP72 immunoreactivity began increasing by 1 hour after hyperthermia and continued to increase thereafter, to reach maximal levels at 6 hours. The maximal levels were maintained for the rest of the experiment--18 hours. The results indicate that hyperthermia leads to an increase in the synthesis of HSP72 in guinea pig interdental cells.
A M Thompson; J G Neely
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery     Volume:  107     ISSN:  0194-5998     ISO Abbreviation:  Otolaryngol Head Neck Surg     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-01-25     Completed Date:  1993-01-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8508176     Medline TA:  Otolaryngol Head Neck Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  769-74     Citation Subset:  IM    
Department of Otorhinolaryngology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
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MeSH Terms
Antibodies, Monoclonal / diagnostic use
Cochlea / metabolism*,  pathology
Fever / metabolism*,  pathology
Guinea Pigs
Heat-Shock Proteins / biosynthesis*
Optics and Photonics
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Heat-Shock Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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