Document Detail

Induction of heat shock protein 70 inhibits NF-kappa-B in squamous cell carcinoma.
MedLine Citation:
PMID:  16025056     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine the relationship between heat shock proteins (HSPs) and the proinflammatory, anti-apoptosis mediator NF-kappa-B in squamous cell carcinoma. STUDY DESIGN AND SETTING: CA-9-22 cells were exposed to heat stress to induce the production of HSPs. Immunoblot and reporter gene experiments determined the inducibility of HSP production and the activation of cytokine-induced NF-kappa-B. Immunoblot experiments determined the presence of the inhibitor-kappa-B-alpha (IkappaB alpha). RESULTS: CA-9-22 cells can be induced by heat stress to produce HSPs at 100-fold above baseline levels. The induction of HSPs prevents the activation and nuclear translocation of NF-kappa-B despite stimulation with IL-1beta and TNF-alpha. CONCLUSIONS: Constitutive activation of NF-kappa-B is prevented by HSP induction through an increase in IkappaB alpha synthesis. SIGNIFICANCE: The induction of HSP70 alters the inflammatory milieu associated with squamous cell carcinoma progression through the inhibition of NF-kappa-B and may ultimately promote apoptosis in head and neck carcinoma.
Timothy A King; Raed Abu Ghazaleh; Steven K Juhn; George L Adams; Frank G Ondrey
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery     Volume:  133     ISSN:  0194-5998     ISO Abbreviation:  Otolaryngol Head Neck Surg     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-18     Completed Date:  2005-09-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8508176     Medline TA:  Otolaryngol Head Neck Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  70-9     Citation Subset:  IM    
Department of Otorhinolaryngology-Head and Neck Surgery, University of Minnesota, Minneapolis 55455, USA.
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MeSH Terms
Apoptosis / physiology
Carcinoma, Squamous Cell / metabolism*,  physiopathology
Cell Line, Tumor
Cell Survival / physiology
HSP70 Heat-Shock Proteins / biosynthesis*
Hot Temperature
Mouth Neoplasms / metabolism*,  physiopathology
NF-kappa B / metabolism*
Stress, Physiological / physiopathology
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/NF-kappa B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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