| Induction of glomerulopressin production by cyclic AMP. | |
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MedLine Citation:
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PMID: 2985485 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Isolated rat livers were perfused with gassed Krebs-Ringer-Bicarbonate and different doses of theophylline and dibutyryl cyclic AMP were added to the perfusing solution. The perfusates were ultrafiltrated through Diaflo UM-05 membranes. The glomerulopressin activity of the ultrafiltrates were assayed in the tonic tension contraction (TTC) of isolated stomach fundus from rats. As glomerulopressin is known to be a glucuronide, it was inactivated with beta-glucuronidase to confirm that the effect on the stomach fundus was due to the glomerulopressin and not to another substance. It was observed that doses of theophylline between 2 x 10(-3) M and 2 x 10(-5) M enhanced glomerulopressin production. However, there was no relationship between dose of theophylline and the response, and a dose of theophylline 2 x 10(-6) M has no activity. The perfusion with dibutyryl cyclic AMP at 5 x 10(-8) M increased the amount of glomerulopressin produced by the liver. This was a log-dose response of glomerulopressin production to dibutyryl cyclic AMP between 5 x 10(-8) M and 5 x 10(-4) M. Theophylline (2 x 10(-6) M) potentiated the activity of cyclic AMP (5 x 10(-8) M). These results support the view that cyclic AMP is intracellular mediator of the hepatic production of glomerulopressin. |
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Authors:
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E Arany; J Uranga; R Bonetto; E del Castillo |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme Volume: 17 ISSN: 0018-5043 ISO Abbreviation: Horm. Metab. Res. Publication Date: 1985 Feb |
Date Detail:
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Created Date: 1985-06-07 Completed Date: 1985-06-07 Revised Date: 2009-02-19 |
Medline Journal Info:
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Nlm Unique ID: 0177722 Medline TA: Horm Metab Res Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 72-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bucladesine / pharmacology* Cyclic AMP / metabolism Glucagon / pharmacology Glucuronates / biosynthesis* Liver / drug effects, metabolism Perfusion Rats Rats, Inbred Strains Theophylline / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Glucuronates; 362-74-3/Bucladesine; 39372-38-8/glomerulopressin; 58-55-9/Theophylline; 60-92-4/Cyclic AMP; 9007-92-5/Glucagon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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