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Induction of genuine autophagy by cationic lipids in mammalian cells.
MedLine Citation:
PMID:  20383065     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Autophagy is a cellular stress response that results in the activation of a lysosomal degradation pathway. In this report, we showed that cationic lipids, a common-used class of transfection reagents, induced genuine autophagy in mammalian cells. Extensive LC3 dot formation was observed by treatment with cationic lipids (with or without DNA), but not neutral lipids, in a HeLa cell line stably expressing GFP-LC3 (HeLa-LC3). Further proofs for autophagy were obtained by the co-localization of the LC3 dots with lysosome-specific staining patterns, observation of LC3-I to LC3-II form conversion and appearance of autophagic vacuoles under TEM. The autophagic flux assay with bafilomycin A1 and degradation of p62/SQSTM1 suggested that the autophagy induced by cationic lipids was primarily due to increased formation of autophagosomes and not decreased turnover. Moreover, cationic lipids induced autophagy in an mTOR-independent manner.
Authors:
Na Man; Yong Chen; Fang Zheng; Wei Zhou; Long-Ping Wen
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Publication Detail:
Type:  Journal Article     Date:  2010-05-16
Journal Detail:
Title:  Autophagy     Volume:  6     ISSN:  1554-8635     ISO Abbreviation:  Autophagy     Publication Date:  2010 May 
Date Detail:
Created Date:  2013-03-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101265188     Medline TA:  Autophagy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  449-54     Citation Subset:  IM    
Affiliation:
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences; University of Science & Technology of China; Hefei, Anhui China.
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