Document Detail

Induction of genomic instability in cultured human colon epithelial cells following exposure to isocyanates.
MedLine Citation:
PMID:  19376255     Owner:  NLM     Status:  MEDLINE    
The toxic response of cultured human colon epithelial-FHC cells to methyl isocyanate was investigated with regard to genomic instability. Qualitative and quantitative assessments of the extent of phosphorylation of DNA damage signaling factors such as ATM, gammaH2AX and p53, was increased in treated cells compared to controls. At the same time, many treated cells were arrested at the G2/M phase of the cell cycle, and had an elevated apoptotic index and increased inflammatory cytokine levels. Cytogenetic analyses revealed varied chromosomal anomalies, with abnormal expression of pericentrin protein. Analysis through ISSR PCR demonstrated increased microsatellite instability. The results imply that isocyanates can cause genomic instability in colonocytes.
Pradyumna Kumar Mishra; Arpit Bhargava; Gorantla Venkata Raghuram; Suresh Kumar Jatawa; Nabila Akhtar; Saba Khan; Archana Tiwari; Kewal Krishan Maudar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-17
Journal Detail:
Title:  Cell biology international     Volume:  33     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-29     Completed Date:  2009-07-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  675-83     Citation Subset:  IM    
Bhopal Memorial Hospital & Research Centre, Bhopal, India.
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MeSH Terms
Antigens / metabolism
Cell Line
Colon / cytology,  drug effects*
Cytokines / metabolism
DNA Damage
Epithelial Cells / drug effects*,  metabolism
G2 Phase
Genomic Instability / drug effects*,  genetics
Isocyanates / pharmacology*
Signal Transduction
Reg. No./Substance:
0/Antigens; 0/Cytokines; 0/Isocyanates; 0/pericentrin; 624-83-9/methyl isocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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