Document Detail


Induction of fat cell necrosis in human fat tissue after treatment with phosphatidylcholine and deoxycholate.
MedLine Citation:
PMID:  22280507     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background  Injections with phosphatidylcholine- and deoxycholate-containing substances are used to treat localized fat accumulation and lipomas. It is believed that the injected substances induce fat cell destruction with subsequent acute panniculitis followed by a repair process of the treated fat tissue. Objectives  We investigated whether necrosis or apoptosis of fat cells was induced by the injected substances. Methods  Samples of fat tissue of lipoma were collected at various times after injection and evaluated by light and electron microscopy, by immunostaining for active caspase-3 and antideoxyribonuclease I, in situ end-labelling (TUNEL staining), and biochemical caspase-3 assays. Results  Light and electron microscopy showed fat cell necrosis in all areas of the treated lipomas. Low levels of active caspase-3 indicated the absence of apoptosis. Conclusions  Injection of the lipolytic substances phosphatidylcholine and deoxycholate leads to fat cell necrosis rather than apoptosis. However, additional studies evaluating different dosing and further time points after treatment are necessary.
Authors:
F G Bechara; H G Mannherz; M Jacob; A J Mazur; M Sand; P Altmeyer; K Hoffmann
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Publication Detail:
Type:  Journal Article     Date:  2011-03-09
Journal Detail:
Title:  Journal of the European Academy of Dermatology and Venereology : JEADV     Volume:  26     ISSN:  1468-3083     ISO Abbreviation:  J Eur Acad Dermatol Venereol     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9216037     Medline TA:  J Eur Acad Dermatol Venereol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  180-5     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. Journal of the European Academy of Dermatology and Venereology © 2011 European Academy of Dermatology and Venereology.
Affiliation:
Departments of Dermatology, Venereology and Allergology Anatomy and Embryology, Ruhr-University Bochum, Bochum, Germany.
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