Document Detail

Induction of estradiol 2-hydroxylase activity in MCF-7 human breast cancer cells by pesticides and carcinogens.
MedLine Citation:
PMID:  21781778     Owner:  NLM     Status:  In-Data-Review    
The induction of 17β-estradiol (E2) 2-hydroxylase activity was investigated in MCF-7 human breast cancer cells using 2-[(3)H]E2 as the substrate in a radiometric assay. Treatment of MCF-7 cells with 10 μM indole-3-carbinol (I3C) for 48 h caused a 3.5-fold induction of E2 2-hydroxylase activity, whereas, I3C at concentrations as high as 100 μM did not induce CYP1A1 mRNA levels or immunoreactive protein. Thus, the induction of E2 2-hydroxylase activity using the radiometric assay was not dependent on induction of CYP1A1. E2 2-hydroxylase activity was also increased by I3C within 2 h after treatment suggesting in situ interactions with the cellular cytochrome P450 system. The time-dependent effects of various chlorinated pesticides, antiestrogens and mammary carcinogens on E2 2-hydroxylase activity were also investigated. p,p'-DDE, atrazine and the mammary carcinogen 7,12-dimethylbenz[a]anthracene (DMBA) significantly decreased E2 2-hydroxylase activity after 2 h; whereas, only the latter two compounds decreased activity after 48 h. Both 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and the mammary carcinogen benzo[a]pyrene (BaP) induced E2 2-hydroxylase in MCF-7 cells after incubation for 48 h and this was also paralleled by induction of CYP1A1 protein. The antiestrogens ICI 164 384 and ICI 182 780 decreased E2 2-hydroxylase activity in MCF-7 cells after incubation for 48 h, whereas tamoxifen and 4-hydroxytamoxifen were inactive. The results indicate that chemical-induced modulation of E2 2-hydroxylase activity in MCF-7 cells is complex and does not predict their activity as mammary carcinogens.
A McDougal; C Wilson; S Safe
Related Documents :
19924738 - Gold nanoparticles capped with polyethyleneimine for enhanced sirna delivery.
18314198 - Single-cell electroporation of adult sensory neurons for gene screening with rna interf...
18278448 - Construction of recombinant adenovirus carrying grim19 and its effect on sw480 cells.
15970688 - Inhibition of basigin expression in glioblastoma cell line via antisense rna reduces tu...
22923218 - Chemokine receptor 7 via proline-rich tyrosine kinase-2 upregulates the chemotaxis and ...
4206868 - Clonal analysis of cell division in the bacillus subtilis div iv-b1 minicell-producing ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Environmental toxicology and pharmacology     Volume:  3     ISSN:  1382-6689     ISO Abbreviation:  Environ. Toxicol. Pharmacol.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  2011-07-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9612020     Medline TA:  Environ Toxicol Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  195-9     Citation Subset:  -    
Veterinary Physiology and Pharmacology, Texas A and M University, College Station, TX 77 843-4466, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cadmium induces the production of high molecular weight heparan sulfate proteoglycan molecules in cu...
Next Document:  Prevention of ozone-induced airway hyperresponsiveness and epithelial injury by phosphodiesterase in...