| Induction of enzyme activity in cell culture: a rapid screen for detection of planar polychlorinated organic compounds. | |
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MedLine Citation:
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PMID: 115836 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Induction of aryl hydrocarbon hydroxylase (AHH) activity in rat hepatoma cell line serves as a simple and rapid method to detect minute (pg) amounts of certain classes of compounds, e.g., dibenzo-p-dioxins, dibenzofurans, and biphenyls. This method may provide a quick screen for such substances in extracts from foods prior to chemical identification. AHH activity is measured by conversion of benzo[a]pyrene (BP) to 3-hydroxy BP in homogenized cell extracts from control and treated cultures and is reported as pmol product formed/mg protein/min. Substances screened by this method include polyhalogenated analogs of dibenzo-p-dioxin (24 compounds), dibenzofuran (11 compounds), biphenyl (7 compounds), and extracts from several food sources. Response of the most reactive compound, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was used to prepare a standard curve, and the AHH activity induced by mole doses of test substance is reported as an ED50 response (the estimated dose needed to produce 50% maximum enzyme induction). The AHH activity induced by food extracts is equated to the standard curve and reported as TCDD equivalents. A potent ED50 response in cell culture appears to correlate well with known toxic responses in other mammalian and avian systems for certain test substances. This correlation suggests that the cell culture enzyme induction method is a useful model for screening food extracts that are suspected to be contaminated with polychlorinated planar substances. A collaborative study would demonstrate the reproducibility of the method. |
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Authors:
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J A Bradlaw; J L Casterline |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal - Association of Official Analytical Chemists Volume: 62 ISSN: 0004-5756 ISO Abbreviation: J Assoc Off Anal Chem Publication Date: 1979 Jul |
Date Detail:
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Created Date: 1980-01-28 Completed Date: 1980-01-28 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7505559 Medline TA: J Assoc Off Anal Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 904-16 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aryl Hydrocarbon Hydroxylases / biosynthesis* Benzofurans / analysis Biological Assay Cells, Cultured Dioxins / analysis Enzyme Induction / drug effects Food Contamination / analysis Hydrocarbons, Chlorinated / analysis* Liver Neoplasms, Experimental / enzymology Polychlorinated Biphenyls / analysis Rats |
| Chemical | |
Reg. No./Substance:
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0/Benzofurans; 0/Dioxins; 0/Hydrocarbons, Chlorinated; 0/Polychlorinated Biphenyls; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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